{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:27:21Z","timestamp":1753878441114,"version":"3.41.2"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,6,7]],"date-time":"2019-06-07T00:00:00Z","timestamp":1559865600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["96\u201001\u201003\u201014816"],"award-info":[{"award-number":["96\u201001\u201003\u201014816"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Invest &amp; Clin Dent"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Incidence of transient bacteremia following dental extractions ranges 30%\u201070% among adults and 58%\u2010100% in children. This study aims to assess the multiplex polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) technique in detection of transient bacteremia during dental extraction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty volunteers for dental extraction entered the study. Blood samples were taken at baseline and 30\u00a0seconds and 15\u00a0minutes after extraction. Five types of bacteria were selected, namely <jats:italic>Streptococcus mutans<\/jats:italic>,<jats:italic> S.\u00a0salivarius<\/jats:italic>,<jats:italic> S.\u00a0sanguinis<\/jats:italic>,<jats:italic> Enterococcus faecalis<\/jats:italic> and <jats:italic>Lactobacillus<\/jats:italic>. Blood samples were evaluated by microbial culture and multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Blood culture showed rates of 0%, 80% and 25% for bacteremia before, during and after dental extraction, respectively. A significant difference was observed between baseline and during extraction. Using <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>, bacteremia was deemed 20%, 100% and 30% before, during and after extraction, respectively, and a significant difference was witnessed between the baseline and during extraction. The highest incidence was at the 30\u2010second mark after extraction, and the most prevalent type of bacteria was <jats:italic>S.\u00a0mutans<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Multiplex <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> can be used to determine bacterial diversity with high accuracy during occurrence of bacteremia. Therefore, in cases where positive blood cultures only indicate the presence of one species, we recommend the use of this method to detect more diverse bacteria types.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jicd.12425","type":"journal-article","created":{"date-parts":[[2019,6,8]],"date-time":"2019-06-08T05:54:55Z","timestamp":1559973295000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Multiplex polymerase chain reaction for detection of bacteremia during dental extraction"],"prefix":"10.1111","volume":"10","author":[{"given":"Azita","family":"Azad","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Medicine Oral and Dental Disease Research Center School of Dentistry Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6253-7629","authenticated-orcid":false,"given":"Farzan","family":"Modaresi","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Jahrom University of Medical Sciences Jahrom Iran"},{"name":"Department of Advanced Medical Sciences and Technology Jahrom University of Medical Sciences Jahrom Iran"},{"name":"Central Laboratory Research Jahrom University of Medical Sciences Jahrom Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Zahed","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Medicine Oral and Dental Disease Research Center School of Dentistry Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahshid","family":"Zarei","sequence":"additional","affiliation":[{"name":"Student Research Committee School of Dentistry Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Ranjbaran","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Medicine Oral and Dental Disease Research Center School of Dentistry Shiraz University of Medical Sciences Shiraz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahra Kargar","family":"Jahrom","sequence":"additional","affiliation":[{"name":"Zoonoses Research Center Jahrom University of Medical Sciences Shiraz Jahrom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,6,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-200504000-00003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2044.2008.05449.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2008.0346"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057782"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2010.10.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.758524"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.00924.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2004.09.008"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00790.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2002.122338"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2008.02.009"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/17.2.188"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.10.2205-2209.1990"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1996.00440050059007"},{"key":"e_1_2_8_16_1","first-page":"28","article-title":"Bacteremia of dental origin and antimicrobial sensitivity following oral surgical procedures in children","volume":"20","author":"Firms BPG","year":"1998","journal-title":"Pediatr Dent"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","unstructured":"Tom\u00e1s\u2010CarmonaI \u00c1lvarez\u2010Fern\u00e1ndezM.Pathogenesis of Endocarditis\u2013Bacteraemia of oral origin. 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