{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:23:03Z","timestamp":1791408183896,"version":"4.3.3"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,3,11]],"date-time":"2014-03-11T00:00:00Z","timestamp":1394496000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31370290"],"award-info":[{"award-number":["31370290"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["30971554"],"award-info":[{"award-number":["30971554"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Agriculture of China","award":["2014ZX0800932B"],"award-info":[{"award-number":["2014ZX0800932B"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Induction and secretion of acid phosphatases (APases) is thought to be an adaptive mechanism that helps plants survive and grow under phosphate (Pi) deprivation. In <jats:italic>Arabidopsis<\/jats:italic>, there are 29 purple acid phosphatase (AtPAP) genes. To systematically investigate the roles of different AtPAPs, we first identified knockout or knock\u2010down T\u2010DNA lines for all 29 <jats:italic>AtPAP<\/jats:italic> genes. Using these <jats:italic>atpap<\/jats:italic> mutants combined with in\u2010gel and quantitative APase enzyme assays, we demonstrated that AtPAP12 and AtPAP26 are two major intracellular and secreted APases in <jats:italic>Arabidopsis<\/jats:italic> while AtPAP10 is mainly a secreted APase. On Pi\u2010deficient (P\u2212) medium or P\u2212 medium supplemented with the organophosphates ADP and fructose\u20106\u2010phosphate (Fru\u20106\u2010P), growth of <jats:italic>atpap10<\/jats:italic> was significantly reduced whereas growth of <jats:italic>atpap12<\/jats:italic> was only moderately reduced, and growth of <jats:italic>atpap26<\/jats:italic> was nearly equal to that of the wild type (WT). Overexpression of the <jats:italic>AtPAP12<\/jats:italic> or <jats:italic>AtPAP26<\/jats:italic> gene, however, caused plants to grow better on P\u2212 or P\u2212 medium supplemented with ADP or Fru\u20106\u2010P. Interestingly, Pi levels are essentially the same for the WT and overexpressing lines, although these two types of plants have significantly different growth phenotypes. These results suggest that the APases may have other roles besides enhancing internal Pi recycling or releasing Pi from external organophosphates for plant uptake.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jipb.12184","type":"journal-article","created":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T11:20:12Z","timestamp":1392636012000},"page":"299-314","source":"Crossref","is-referenced-by-count":126,"title":["Comparative genetic analysis of <i>Arabidopsis<\/i> purple acid phosphatases AtPAP10, AtPAP12, and AtPAP26 provides new insights into their roles in plant adaptation to phosphate deprivation"],"prefix":"10.1111","volume":"56","author":[{"given":"Liangsheng","family":"Wang","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Bioinformatics, Center for Plant Biology School of Life Sciences, Tsinghua University Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Lu","sequence":"additional","affiliation":[{"name":"MOE 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