{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:28:38Z","timestamp":1789644518295,"version":"4.0.0"},"reference-count":40,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T00:00:00Z","timestamp":1426464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Italian Ministry of Foreign Affairs","award":["PGR00348"],"award-info":[{"award-number":["PGR00348"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p><jats:italic>Arabidopsis<\/jats:italic> <jats:italic>abcb1<\/jats:italic> <jats:italic>abcb19<\/jats:italic> double mutants defective in the auxin transporters ABCB1\/PGP1 and ABCB19\/PGP19 are altered in stamen elongation, anther dehiscence and pollen maturation. To assess the contribution of these transporters to stamen development we performed phenotypic, histological analyses, and <jats:italic>in situ<\/jats:italic> hybridizations on <jats:italic>abcb1<\/jats:italic> and <jats:italic>abcb19<\/jats:italic> single mutant flowers. We found that pollen maturation and anther dehiscence are precocious in the <jats:italic>abcb1<\/jats:italic> but not in the <jats:italic>abcb19<\/jats:italic> mutant. Accordingly, endothecium lignification is altered only in <jats:italic>abcb1<\/jats:italic>  anthers. Both <jats:italic>abcb1<\/jats:italic> and <jats:italic>abcb1 abcb19<\/jats:italic>  stamens also show altered early development, with asynchronous anther locules and a multilayer tapetum. DAPI staining showed that the timing of meiosis is asynchronous in <jats:italic>abcb1 abcb19<\/jats:italic>  anther locules, while only a small percentage of pollen grains are non\u2010viable according to Alexander's staining. In agreement, <jats:italic>TAM<\/jats:italic> (TARDY ASYNCHRONOUS MEIOSIS), as well as <jats:italic>BAM2<\/jats:italic> (BARELY ANY MERISTEM)\u2014involved in tapetal cell development\u2014are overexpressed in <jats:italic>abcb1 abcb19<\/jats:italic>  young flower buds. Correspondingly, <jats:italic>ABCB1<\/jats:italic> and <jats:italic>ABCB19<\/jats:italic> mRNA localization supports the observed phenotypes of <jats:italic>abcb1<\/jats:italic> and <jats:italic>abcb1 abcb19<\/jats:italic> mutant anthers. In conclusion, we provide evidence that auxin transport plays a significant role both in early and late stamen development: <jats:italic>ABCB1<\/jats:italic>  plays a major role during anther development, while <jats:italic>ABCB19<\/jats:italic> has a synergistic role.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jipb.12332","type":"journal-article","created":{"date-parts":[[2015,1,27]],"date-time":"2015-01-27T11:34:45Z","timestamp":1422358485000},"page":"1089-1098","source":"Crossref","is-referenced-by-count":73,"title":["ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late <i>Arabidopsis<\/i> anther development"],"prefix":"10.1111","volume":"57","author":[{"given":"Valentina","family":"Cecchetti","sequence":"first","affiliation":[{"name":"IBPM\u2010CNR Institute of Molecular Biology and Pathology National Research Council Sapienza University of Rome Rome 00185 Italy"},{"name":"Department of Biology and Biotechnology Sapienza University of Rome Rome 00185 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrizia","family":"Brunetti","sequence":"additional","affiliation":[{"name":"IBPM\u2010CNR Institute of Molecular Biology and Pathology National Research Council Sapienza University of Rome Rome 00185 Italy"},{"name":"Department of Biology and Biotechnology Sapienza University of Rome Rome 00185 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Napoli","sequence":"additional","affiliation":[{"name":"IBPM\u2010CNR Institute of Molecular Biology and Pathology National Research Council Sapienza University of Rome Rome 00185 Italy"},{"name":"Department of Biology and Biotechnology Sapienza University of Rome Rome 00185 Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Fattorini","sequence":"additional","affiliation":[{"name":"Department of Environmental Biology Sapienza University of Rome Rome 00185 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