{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:44:46Z","timestamp":1790858686703,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP15J03980"],"award-info":[{"award-number":["JP15J03980"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Previously, we found 123 transcription factors (TFs) as candidate regulators of secondary cell wall (SCW) formation in rice by using phylogenetic and co\u2010expression network analyses. Among them, we examined in this work the role of OsIDD2, a zinc finger and indeterminate domain (IDD) family TF. Its overexpressors showed dwarfism, fragile leaves, and decreased lignin content, which are typical phenotypes of plants defective in SCW formation, whereas its knockout plants showed slightly increased lignin content. The RNA\u2010seq and quantitative reverse transcription polymerase chain reaction analyses confirmed that some lignin biosynthetic genes were downregulated in the OsIDD2\u2010overexpressing plants, and revealed the same case for other genes involved in cellulose synthesis and sucrose metabolism. The transient expression assay using rice protoplasts revealed that OsIDD2 negatively regulates the transcription of genes involved in lignin biosynthesis, <jats:italic>cinnamyl alcohol dehydrogenase 2<\/jats:italic> and <jats:italic>3<\/jats:italic> (<jats:italic>CAD2<\/jats:italic> and <jats:italic>3<\/jats:italic>), and sucrose metabolism, <jats:italic>sucrose synthase 5<\/jats:italic> (<jats:italic>SUS5<\/jats:italic>), whereas an AlphaScreen assay, which can detect the interaction between TFs and their target DNA sequences, directly confirmed the interaction between OsIDD2 and the target sequences located in the promoter regions of <jats:italic>CAD2<\/jats:italic> and <jats:italic>CAD3<\/jats:italic>. Based on these observations, we conclude that OsIDD2 is negatively involved in SCW formation and other biological events by downregulating its target genes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jipb.12557","type":"journal-article","created":{"date-parts":[[2017,6,2]],"date-time":"2017-06-02T11:22:53Z","timestamp":1496402573000},"page":"130-143","source":"Crossref","is-referenced-by-count":47,"title":["OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation"],"prefix":"10.1111","volume":"60","author":[{"given":"Peng","family":"Huang","sequence":"first","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 Japan"},{"name":"State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Yano","sequence":"additional","affiliation":[{"name":"Graduate School of Agricultural and Life Sciences The University of Tokyo Tokyo 113\u20108657 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Kinoshita","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyosuke","family":"Kawai","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eriko","family":"Koketsu","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 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Lacsamana","family":"Ordonio","sequence":"additional","affiliation":[{"name":"Plant Breeding and Biotechnology Division Philippine Rice Research Institute Maligaya Science City of Munoz 3119 The Philippines"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyako","family":"Ueguchi\u2010Tanaka","sequence":"additional","affiliation":[{"name":"Bioscience and Biotechnology Center Nagoya University Nagoya 464\u20108601 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