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FYVE DOMAIN PROTEIN REQUIRED FOR ENDOSOMAL SORTING 1 (FREE1), which was previously identified as a plant\u2010specific ESCRT component, modulates MVB\u2010mediated endosomal sorting and autophagic degradation. Although the basic cellular functions of FREE1 as an ESCRT component have been described, the regulators that control FREE1 turnover remain unknown. Here, we analyzed how FREE1 homeostasis is mediated by the RING\u2010finger E3 ubiquitin ligases, SINA of <jats:italic>Arabidopsis thaliana<\/jats:italic> (SINATs), in response to iron deficiency. Under iron\u2010deficient growth conditions, SINAT1\u20104 were induced and ubiquitinated FREE1, thereby promoting its degradation and relieving the repressive effect of FREE1 on iron absorption. By contrast, SINAT5, another SINAT member that lacks ubiquitin ligase activity due to the absence of the RING domain, functions as a protector protein which stabilizes FREE1. Collectively, our findings uncover a hitherto unknown mechanism of homeostatic regulation of FREE1, and demonstrate a unique regulatory SINAT\u2013FREE1 module that subtly regulates plant response to iron deficiency stress.<\/jats:p>","DOI":"10.1111\/jipb.13005","type":"journal-article","created":{"date-parts":[[2020,8,12]],"date-time":"2020-08-12T19:24:19Z","timestamp":1597260259000},"page":"1399-1417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["SINAT E3 ligases regulate the stability of the ESCRT component FREE1 in response to iron deficiency in plants"],"prefix":"10.1111","volume":"62","author":[{"given":"Zhidan","family":"Xiao","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences South China Normal University  Guangzhou 510631 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