{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T20:05:47Z","timestamp":1791576347295,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Iron (Fe) homeostasis is critical for plant growth, development, and stress responses. Fe levels are tightly controlled by intricate regulatory networks in which transcription factors (TFs) play a central role. A series of basic helix\u2010loop\u2010helix (bHLH) TFs have been shown to contribute to Fe homeostasis, but the regulatory layers beyond bHLH TFs remain largely unclear. Here, we demonstrate that the SQUAMOSA PROMOTER\u2010BINDING PROTEIN\u2010LIKE (SPL) TF SlSPL\u2010CNR negatively regulates Fe\u2010deficiency responses in tomato (\n                    <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    ) roots. Fe deficiency rapidly repressed the expression of\n                    <jats:italic>SlSPL\u2010CNR<\/jats:italic>\n                    , and Fe deficiency responses were intensified in two clustered regularly interspaced palindromic repeats (CRISPR)\/CRISPR\u2010associated protein 9\u2010generated SlSPL\u2010CNR knock\u2010out lines compared to the wild\u2010type. Comparative transcriptome analysis identified 47 Fe deficiency\u2010responsive genes the expression of which is negatively regulated by SlSPL\u2010CNR, one of which,\n                    <jats:italic>SlbHLH101<\/jats:italic>\n                    , helps regulate Fe uptake genes. SlSPL\u2010CNR localizes the nucleus and interacts with the GTAC and BOX 4 (ATTAAT) motifs in the\n                    <jats:italic>SlbHLH101<\/jats:italic>\n                    promoter to repress its expression. Inhibition of\n                    <jats:italic>SlSPL\u2010CNR<\/jats:italic>\n                    expression in response to Fe deficiency was well correlated with the expression of the microRNA\n                    <jats:italic>SlymiR157<\/jats:italic>\n                    .\n                    <jats:italic>SlymiR157<\/jats:italic>\n                    \u2010overexpressing tomato lines displayed enhanced Fe deficiency responses, as did\n                    <jats:italic>SlSPL\u2010CNR<\/jats:italic>\n                    loss\u2010of\u2010function mutants. We propose that the SlymiR157\u2010SlSPL\u2010CNR module represents a novel pathway that acts upstream of SlbHLH101 to regulate Fe homeostasis in tomato roots.\n                  <\/jats:p>","DOI":"10.1111\/jipb.13251","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T04:59:20Z","timestamp":1647493160000},"page":"1059-1075","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["The miR157\u2010SPL\u2010CNR module acts upstream of bHLH101 to negatively regulate iron deficiency responses in tomato"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0955-0263","authenticated-orcid":false,"given":"Huihui","family":"Zhu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences Zhejiang University  Hangzhou 310058 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