{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:49:29Z","timestamp":1791244169388,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,12,31]],"date-time":"2022-12-31T00:00:00Z","timestamp":1672444800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>It is of great importance to better understand how trees regulate nitrogen (N) uptake under N deficiency conditions which severely challenge afforestation practices, yet the underlying molecular mechanisms have not been well elucidated. Here, we functionally characterized PuHox52, a <jats:italic>Populus ussuriensis<\/jats:italic> HD\u2010ZIP transcription factor, whose overexpression greatly enhanced nutrient uptake and plant growth under N deficiency. We first conducted an RNA sequencing experiment to obtain root transcriptome using <jats:italic>PuHox52<\/jats:italic>\u2010overexpression lines of <jats:italic>P. ussuriensis<\/jats:italic> under low N treatment. We then performed multiple genetic and phenotypic analyses to identify key target genes of PuHox52 and validated how they acted against N deficiency under PuHox52 regulation. <jats:italic>PuHox52<\/jats:italic> was specifically induced in roots by N deficiency, and overexpression of <jats:italic>PuHox52<\/jats:italic> promoted N uptake, plant growth, and root development. We demonstrated that several nitrate\u2010responsive genes (<jats:italic>PuNRT1.1<\/jats:italic>, <jats:italic>PuNRT2.4<\/jats:italic>, <jats:italic>PuCLC\u2010b<\/jats:italic>, <jats:italic>PuNIA2<\/jats:italic>, <jats:italic>PuNIR1<\/jats:italic>, and <jats:italic>PuNLP1<\/jats:italic>), phosphate\u2010responsive genes (<jats:italic>PuPHL1A<\/jats:italic> and <jats:italic>PuPHL1B<\/jats:italic>), and an iron transporter gene (<jats:italic>PuIRT1<\/jats:italic>) were substantiated to be direct targets of PuHox52. Among them, <jats:italic>PuNRT1.1<\/jats:italic>, <jats:italic>PuPHL1A<\/jats:italic>\/<jats:italic>B<\/jats:italic>, and <jats:italic>PuIRT1<\/jats:italic> were upregulated to relatively higher levels during PuHox52\u2010mediated responses against N deficiency in <jats:italic>PuHox52<\/jats:italic>\u2010overexpression lines compared to WT. Our study revealed a novel regulatory mechanism underlying root adaption to N deficiency where PuHox52 modulated a coordinated uptake of nitrate, phosphate, and iron through \u2018PuHox52\u2010PuNRT1.1\u2019, \u2018PuHox52\u2010PuPHL1A\/PuPHL1B\u2019, and \u2018PuHox52\u2010PuIRT1\u2019 regulatory relationships in poplar roots.<\/jats:p>","DOI":"10.1111\/jipb.13389","type":"journal-article","created":{"date-parts":[[2022,10,13]],"date-time":"2022-10-13T09:09:12Z","timestamp":1665652152000},"page":"791-809","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["PuHox52 promotes coordinated uptake of nitrate, phosphate, and iron under nitrogen deficiency in <i>Populus ussuriensis<\/i>"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0793-3028","authenticated-orcid":false,"given":"Ming","family":"Wei","sequence":"first","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, School of Forestry Northeast Forestry University Harbin 150040 China"},{"name":"College of Life Sciences Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengqiu","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, School of Forestry Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiali","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, School of Forestry Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, School of Forestry Northeast Forestry University Harbin 150040 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingli","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, School of Forestry Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hairong","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Forest Resources and Environmental Science Michigan Technological University Houghton Michigan 49931 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0405-2823","authenticated-orcid":false,"given":"Yuhua","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northeast Forestry University Harbin 150040 China"},{"name":"Key Laboratory of Saline\u2010alkali Vegetation Ecology Restoration (Northeast Forestry University) Ministry of Education Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0916-0409","authenticated-orcid":false,"given":"Chenghao","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Tree Genetics and Breeding, School of Forestry Northeast Forestry University Harbin 150040 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200603000-00009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.15"},{"key":"e_1_2_8_4_1","first-page":"1237","article-title":"Nitrate controls root development through posttranscriptional regulation of the NRT1.1\/NPF6.3 transporter\/sensor","volume":"172","author":"Bouguyon E.","year":"2016","journal-title":"Plant 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