{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T04:58:24Z","timestamp":1758862704081,"version":"3.37.3"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T00:00:00Z","timestamp":1734048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Plant growth is determined by the production of cells and initiation of new organs. Exploring genes that control cell number and cell size is of great significance for understanding plant growth regulation. In this study, we characterized two wheat mutants, <jats:italic>ah<\/jats:italic> and <jats:italic>dl<\/jats:italic>, with abnormal growth. The <jats:italic>ah<\/jats:italic> mutant is a naturally occurring variant characterized by severe dwarfism, increased tiller number, and reduced grain length, while the <jats:italic>dl<\/jats:italic> mutant is derived from an ethyl methane sulfonate (EMS)\u2010mutagenized population and exhibits smaller grain size and slightly reduced plant height. Cytological analyses revealed abnormal cell number, cell morphology and arrangement in the stems and leaves of the <jats:italic>ah<\/jats:italic> mutant, along with reduced cell length in the grains of the dl mutant. Map\u2010based cloning identified that both mutants carry mutations in the same gene <jats:italic>TavWA1\u20107D<\/jats:italic>, which encodes a protein with a von Willebrand factor A (vWA) domain. The <jats:italic>ah<\/jats:italic> mutant harbors a 174\u2010bp insertion in the 1,402\u2010bp coding sequence (CDS) of <jats:italic>TavWA1\u20107D<\/jats:italic>, causing premature termination of protein translation, while the <jats:italic>dl<\/jats:italic> mutant contains a Glu420Lys substitution. Mimicking the <jats:italic>TavWA1\u20107D<\/jats:italic><jats:sup><jats:italic>ah<\/jats:italic><\/jats:sup> through clustered regularly interspaced short palindromic repeats (CRISPR)\/CRISPR\u2010associated nuclease 9\u2010mediated genome editing leads to a severe dwarfism phenotype. The C\u2010terminus of the protein is crucial for its correct subcellular localization and interaction, supporting its critical role for TavWA1\u20107D function. Proteomic analysis showed that the dwarf phenotype of the <jats:italic>ah<\/jats:italic> mutant is associated with impaired photosynthesis, ribosome function, and nucleosome formation. Additionally, TavWA1\u20107D interacts with an E3 ligase, TaVIP1\u20103B, the expression levels of which are elevated in both mutants. Overexpression and knockout studies of <jats:italic>TaVIP1\u20103B<\/jats:italic> demonstrated its negative regulatory role in cell length and grain size. Together, our findings suggest that TavWA1\u20107D plays a vital role in regulating wheat growth and yield\u2010related traits, with the <jats:italic>dl<\/jats:italic> mutant's short grain phenotype being associated with TaVIP1\u20103B expression levels.<\/jats:p>","DOI":"10.1111\/jipb.13807","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T12:00:46Z","timestamp":1734091246000},"page":"71-86","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>TavWA1<\/i> is critical for wheat growth by modulating cell morphology and arrangement"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3875-5724","authenticated-orcid":false,"given":"Guowei","family":"Chang","sequence":"first","affiliation":[{"name":"Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design Chinese Academy of Sciences 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