{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:49:28Z","timestamp":1792068568708,"version":"4.4.0"},"reference-count":66,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Jasmonates (JAs) are vital to plant defense and stress adaptation. In\n                    <jats:italic>Salvia miltiorrhiza<\/jats:italic>\n                    , JA significantly promotes tanshinone accumulation, a class of bioactive diterpenoids. Although transcriptional regulation of JA signaling is well characterized, the post\u2010translational mechanisms governing JA\u2010mediated tanshinone biosynthesis remain poorly understood. Here, we identify SmWRKY2 as a critical regulator of JA\u2010induced tanshinone accumulation, which interacts with SmCPK20. SmCPK20 positively regulates JA\u2010induced tanshinone accumulation. JA signaling activates SmCPK20, promoting phosphorylation of SmWRKY2 at Thr\u2010256. This phosphorylation event significantly enhances SmWRKY2's binding to the\n                    <jats:italic>SmCPS1<\/jats:italic>\n                    promoter and its transcriptional activation capacity, thereby promoting tanshinone accumulation. Furthermore, SmWRKY2 interacts with the JA signaling repressor SmJAZ3. SmJAZ3 inhibits the SmWRKY2\u2013SmCPK20 interaction in the absence of JA. JA\u2010triggered degradation of SmJAZ3 releases SmWRKY2, enabling SmCPK20\u2010mediated phosphorylation and promoting tanshinone biosynthesis. Collectively, our results demonstrate that JA and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    signaling pathways cooperate to regulate plant secondary metabolism.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70195","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:16:33Z","timestamp":1772990193000},"page":"1883-1897","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["SmCPK20 phosphorylates SmWRKY2 to regulate jasmonate\u2010mediated tanshinone synthesis in\n                    <i>Salvia miltiorrhiza<\/i>"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1134-747X","authenticated-orcid":false,"given":"Xueying","family":"Li","sequence":"first","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2489-3962","authenticated-orcid":false,"given":"Xiumin","family":"Nie","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3571-1936","authenticated-orcid":false,"given":"Bolun","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4072-7103","authenticated-orcid":false,"given":"Xi","family":"Han","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4254-4789","authenticated-orcid":false,"given":"Xiaona","family":"Cai","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4604-6305","authenticated-orcid":false,"given":"Qian","family":"Yao","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4443-1597","authenticated-orcid":false,"given":"Junwen","family":"Bu","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5536-5283","authenticated-orcid":false,"given":"Jiulin","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Chemistry &amp; Environment Ankang University Ankang 725000 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0660-0375","authenticated-orcid":false,"given":"Bin","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0144-6982","authenticated-orcid":false,"given":"Juane","family":"Dong","sequence":"additional","affiliation":[{"name":"College of Life Sciences Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20112072"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2024272118"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16793"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.00554"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koad126"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.111740"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14373"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-021-00652-6"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.820582"},{"key":"e_1_2_8_11_1","first-page":"1607","article-title":"Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza","volume":"169","author":"Cui G.","year":"2015","journal-title":"Plant Physiol."},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c02500"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18304"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2019.03.007"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erad302"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad066"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19602"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00810"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2023.04.002"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17453"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003127"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.13790"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiaf219"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/bab.1236"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13493"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2021.11.012"},{"key":"e_1_2_8_27_1","first-page":"7805","article-title":"Characterization, expression profiling, and functional identification of a gene encoding geranylgeranyl diphosphate synthase from Salvia miltiorrhiza","volume":"63","author":"Kai G.","year":"2010","journal-title":"J. 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