{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:11:40Z","timestamp":1788613900535,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Dactylobotrys graminicola<\/jats:italic>\n                    (\n                    <jats:italic>Dgr<\/jats:italic>\n                    ), a necrotrophic fungal pathogen, has recently been identified as the causative agent of a novel sheath\u2010rot disease in hulless barley, causing substantial reductions in yield and quality. Research has mainly focused on early detection and fungicide screening, while the resistance mechanisms in hulless barley remain poorly understood. This knowledge gap substantially impedes the breeding of resistant varieties and the development of environmentally sustainable control technologies. In this study, RNA sequencing and metabolomic analyses were conducted on hulless barley varieties exhibiting contrasting resistance to\n                    <jats:italic>Dgr<\/jats:italic>\n                    infection. These analyses revealed that the phenylpropanoid biosynthesis pathway was markedly upregulated in the resistant cultivar Z257. Notably, accumulation and secretion of coniferyl aldehyde (CA) were elevated in Z257 compared to the susceptible cultivar Z251.\n                    <jats:italic>In vitro<\/jats:italic>\n                    antifungal assays demonstrated that CA strongly inhibited\n                    <jats:italic>Dgr<\/jats:italic>\n                    growth. Drug affinity responsive target stability and microscale thermophoresis assays identified pyruvate kinase (PK) as CA's target. Molecular docking and enzymatic activity assays indicated that CA disrupts the binding of the substrate phosphoenolpyruvate to the active site of PK, reducing enzymatic activity. In conclusion, hulless barley enhances its resistance against\n                    <jats:italic>Dgr<\/jats:italic>\n                    by secreting CA, which inhibits the pathogen's PK, establishing an effective defense mechanism.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70271","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:06:48Z","timestamp":1777378008000},"page":"3490-3507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Coniferyl aldehyde from the phenylpropanoid pathway targets pyruvate kinase in\n                    <i>Dactylobotrys graminicola<\/i>\n                    to confer sheath rot resistance in hulless barley"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7091-8746","authenticated-orcid":false,"given":"Haowen","family":"Zheng","sequence":"first","affiliation":[{"name":"Academy of Agriculture and Forestry Science Qinghai University Xining 810016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1771-585X","authenticated-orcid":false,"given":"Liyifan","family":"Chen","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Science Qinghai University Xining 810016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7558-8939","authenticated-orcid":false,"given":"Jianguo","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1519-769X","authenticated-orcid":false,"given":"Yuandi","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5616-0301","authenticated-orcid":false,"given":"Rangrang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4894-1574","authenticated-orcid":false,"given":"Yu","family":"Li","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9827-4171","authenticated-orcid":false,"given":"Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Science Qinghai University Xining 810016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8335-1895","authenticated-orcid":false,"given":"Jiahui","family":"Yan","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Science Qinghai University Xining 810016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3881-9924","authenticated-orcid":false,"given":"Lu","family":"Hou","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Science Qinghai University Xining 810016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3054-5189","authenticated-orcid":false,"given":"Yiliang","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Chemistry and Chemical Engineering Anhui University of Technology Ma'anshan 243032 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5575-0122","authenticated-orcid":false,"given":"Zhensheng","family":"Kang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Northwest A&amp;F University Yangling 712100 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7963-9071","authenticated-orcid":false,"given":"Haifeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Nanjing Agricultural University Nanjing 210095 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8873-1737","authenticated-orcid":false,"given":"Qiang","family":"Yao","sequence":"additional","affiliation":[{"name":"Academy of Agriculture and Forestry Science Qinghai University Xining 810016 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-025-10176-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16223"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2007.01770.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koad266"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.3.6.1525-1532.2004"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11162605"},{"key":"e_1_2_9_8_1","first-page":"213","article-title":"Genetic diversity of Dactylobotrys graminicola and its pathogenicity to Hordeum vulgare var. nudum seedlings","volume":"53","author":"Chen L.","year":"2020","journal-title":"Sci. Agric. Sin."},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c00707"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-025-01170-6"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06734"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e13810"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-022-10402-y"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.postharvbio.2023.112633"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c07022"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-021-03630-9"},{"key":"e_1_2_9_17_1","first-page":"331","article-title":"Dactylobotrys graminicola: A new phytopathogen causing sheath rot of hulless barley and oats","volume":"34","author":"He SuQin H.S.","year":"2015","journal-title":"Mycosystema"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2023.154078"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2501-2507.1994"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14102314"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-020-0541-x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(98)00021-5"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm882"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3317"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10051064"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242417552"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.15592"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-024-02008-3"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.ads0377"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.110065"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12030248"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-64487-2"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.00266"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13082"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.70129"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2505532122"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.70245"},{"key":"e_1_2_9_39_1","first-page":"1","volume-title":"Introduction to plant secondary metabolites. In Vitro Production of Plant Secondary Metabolites: Theory and Practice","author":"Umalatha G.","year":"2025"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14865"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.5c06966"},{"key":"e_1_2_9_42_1","first-page":"281","article-title":"Biological characteristics of Dactylobotrys graminicola and fungicides screening test in vitro","volume":"37","author":"Wang S.","year":"2017","journal-title":"J. Triticeae Crops"},{"key":"e_1_2_9_43_1","doi-asserted-by":"crossref","DOI":"10.1002\/advs.202509002","article-title":"MoMtg1 zcts as a novel transcriptional repressor of MoSwi6 during appressorium\u2010mediated penetration in the rice blast fungus","volume":"12","author":"Wang X.","year":"2025","journal-title":"Adv. Sci."},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14303"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-10-10-0229"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-76921-w"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.8b03797"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/09540105.2019.1603287"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adq5329"}],"container-title":["Journal of Integrative Plant Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jipb.70271","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jipb.70271","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jipb.70271","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:15:26Z","timestamp":1788610526000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jipb.70271"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,28]]},"references-count":48,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/jipb.70271"],"URL":"https:\/\/doi.org\/10.1111\/jipb.70271","archive":["Portico"],"relation":{},"ISSN":["1672-9072","1744-7909"],"issn-type":[{"value":"1672-9072","type":"print"},{"value":"1744-7909","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,28]]},"assertion":[{"value":"2025-12-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}