{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:57:37Z","timestamp":1790859457093,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T00:00:00Z","timestamp":1779667200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Rising global temperature threatens fruit quality. As one of the most economically valuable fruits worldwide, citrus suffers from high\u2010temperature\u2010induced chlorophyll retention during ripening, significantly reducing commercial value. Comparative physiological analyses revealed that citrus fruit stored at 30\u00b0C exhibited markedly impaired chlorophyll degradation and failed to undergo normal degreening, in stark contrast to those maintained at 20\u00b0C. This temperature\u2010dependent suppression of degreening was closely associated with a significant downregulation of key chlorophyll catabolic genes (CCGs). By integrating weighted gene co\u2010expression network analysis (WGCNA) of a stay\u2010green mutant, we identified the transcription factor CsbHLH60, a nuclear\u2010localized transcriptional activator, from a chlorophyll\u2010associated module. CsbHLH60 directly binds E\u2010box motifs in the promoters of\n                    <jats:italic>CsSGR<\/jats:italic>\n                    and\n                    <jats:italic>CsRCCR<\/jats:italic>\n                    to drive their expression, a finding validated by ChIP\u2010qPCR and EMSA assays. Transient overexpression of\n                    <jats:italic>CsbHLH60<\/jats:italic>\n                    in citrus peel accelerated chlorophyll degradation and, importantly, overcame high\u2010temperature\u2010induced repression to restore degreening, whereas RNAi\u2010mediated silencing at 20\u00b0C significantly impeded normal degreening of the peel. Mechanistically, high temperature imposes a dual \u201cdouble\u2010lock\u201d suppression on CsbHLH60, concurrently repressing its transcription and promoting its protein degradation, thereby depleting functional activator levels. Collectively, our findings establish CsbHLH60 as a pivotal temperature\u2010responsive regulator that directly couples temperature signaling to chlorophyll catabolism and is capable of overriding the high\u2010temperature\u2010induced suppression of chlorophyll degradation, thereby highlighting its potential as a molecular target for mitigating climate\u2010driven declines in citrus fruit appearance quality.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70297","type":"journal-article","created":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T10:01:48Z","timestamp":1779703308000},"page":"3790-3808","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The transcription factor CsbHLH60 relieves high\u2010temperature inhibition of chlorophyll degradation in citrus"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8040-776X","authenticated-orcid":false,"given":"Zhehui","family":"Zhang","sequence":"first","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8780-1846","authenticated-orcid":false,"given":"Yongjun","family":"Hu","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1587-9524","authenticated-orcid":false,"given":"Ming","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-8650-0257","authenticated-orcid":false,"given":"Ziyi","family":"Lei","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops Huazhong Agricultural University Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7570-7133","authenticated-orcid":false,"given":"Shengcai","family":"Liao","sequence":"additional","affiliation":[{"name":"Zigui County Fruit and Tea Industry Development Center Yichang 443600 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6171-9814","authenticated-orcid":false,"given":"Liping","family":"Li","sequence":"additional","affiliation":[{"name":"Zigui County Fruit and Tea Industry Development Center Yichang 443600 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5852-4315","authenticated-orcid":false,"given":"Xiongjie","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops Huazhong Agricultural University Wuhan 430070 China"},{"name":"Hubei Hongshan Laboratory Wuhan 430070 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9508-175X","authenticated-orcid":false,"given":"Kaijie","family":"Zhu","sequence":"additional","affiliation":[{"name":"National Key Laboratory for Germplasm Innovation &amp; Utilization of Horticultural Crops Huazhong Agricultural University Wuhan 430070 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