{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T08:24:31Z","timestamp":1786955071371,"version":"build-2736575974"},"reference-count":62,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The early morphogenesis of the embryo and endosperm sets the upper limit of rice grain yield; yet, the cellular dynamics and regulatory mechanisms underlying this critical stage remain largely elusive. Here, we present a single\u2010cell atlas of developing rice seeds based on single\u2010nucleus RNA sequencing of 67,922 high\u2010quality nuclei from rice caryopses. Integration with bulk RNA\u2010seq,\n                    <jats:italic>in situ<\/jats:italic>\n                    hybridization, and promoter\u2010GUS staining enabled systematic cell\u2010type annotation and revealed a distinct population of embryo\u2013endosperm interface (EEI) cells occupying the boundary between the developing embryo and endosperm. Comparative analyses with maize data sets, together with trajectory inference, suggested that a subset of EEI cells shares molecular features with maize embryo\u2010adjacent scutellum cells and shows transcriptional continuity with starchy endosperm cells. Embryo\u2013endosperm interface cells were enriched in transport\u2010related and developmental regulatory genes with known functions in seed development and embryogenesis. Focusing on the EEI\u2010enriched regulator OsBZR4, we found that loss of\n                    <jats:italic>OsBZR4<\/jats:italic>\n                    altered cellular composition and transcriptional programs during early seed development, disrupted embryonic developmental progression, and reduced the expression of embryonic genes, including\n                    <jats:italic>OsCDP3.10<\/jats:italic>\n                    and\n                    <jats:italic>RINO1<\/jats:italic>\n                    . Together, our study provides a single\u2010cell resolution framework for understanding early rice seed development, identifies the embryo\u2013endosperm interface as an important cellular domain associated with embryogenesis, and offers a valuable resource for dissecting the molecular basis of seed formation in rice and related cereals.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70373","type":"journal-article","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T07:38:12Z","timestamp":1786952292000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A single\u2010nucleus transcriptomic atlas reveals distinct cell identities and key regulators of cellular differentiation during early rice seed development"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4006-0695","authenticated-orcid":false,"given":"Yingxiang","family":"Liu","sequence":"first","affiliation":[{"name":"Northeast 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