{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T13:11:33Z","timestamp":1788613893850,"version":"build-2803163510"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32525052"],"award-info":[{"award-number":["32525052"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The prime editing (PE) system is a precise genome editing technology that works efficiently in monocots; however, its application is limited by low editing efficiency in dicots, particularly Cucurbitaceae and Solanaceae plants. Here, we first significantly improved the transformation efficiency by introducing spectinomycin in cucurbits, then used the tomato elongation factor 1\u2010alpha (\n                    <jats:italic>SlEF1\u03b1<\/jats:italic>\n                    ) promoter to enhance PE protein expression, and incorporated the Csy4 ribonuclease to process pegRNAs, collectively addressing multiple constraints limiting PE efficiency in cucurbits. The optimized PE systems, particularly Csy4\u2010PE6d, achieved an average desired editing frequency of 80.83% at targeted loci in cucumber via stable genetic transformation, with frequencies reaching up to 100% at certain sites. Moreover, Csy4\u2010PE6d generated homozygous edits in 36.43% of transgenic lines and demonstrated robust editing activity in melon, pumpkin, and potato. Using the Csy4\u2010PE6d tool, we generated heritable edited cucumber lines with dual resistance to bacterial angular leaf spot and downy mildew by targeting the\n                    <jats:italic>CsSGR<\/jats:italic>\n                    gene. Collectively, this optimized system substantially enhances PE efficiency in Cucurbit crops, providing an effective solution to common challenges such as low editing efficiency and limited heritability in these species.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70375","type":"journal-article","created":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T07:22:57Z","timestamp":1787124177000},"page":"3340-3351","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development of a highly efficient prime editing platform for cucurbits enables breeding of multi\u2010disease\u2010resistant cucumber"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3424-7402","authenticated-orcid":false,"given":"Junya","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7439-0289","authenticated-orcid":false,"given":"Ling","family":"Xiao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0318-8446","authenticated-orcid":false,"given":"Tongxu","family":"Xin","sequence":"additional","affiliation":[{"name":"Anhui Province Key laboratory of Horticultural Crop Quality Biology, School of Horticulture Anhui Agricultural University Hefei 230036 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-4966-6235","authenticated-orcid":false,"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"Anhui Province Key Laboratory of Rice Germplasm Innovation and Molecular Improvement Rice Research Institute, Anhui Academy of Agricultural Sciences Hefei 230031 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0453-2023","authenticated-orcid":false,"given":"Xueyong","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Beijing 100081 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X. Hu Y. Han J. Zhao L. Jin Z. Xu X. Feng J. andWen Y.(2025).Generation of a collection of MYB mutant lines via pooled CRISPR\u2010Cas9 in grape. J. Integr. Agric.24:3287\u20133290.","DOI":"10.1016\/j.jia.2024.12.038"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-024-01898-3"}],"container-title":["Journal of Integrative Plant Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jipb.70375","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jipb.70375","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jipb.70375","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T12:15:13Z","timestamp":1788610513000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jipb.70375"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1111\/jipb.70375"],"URL":"https:\/\/doi.org\/10.1111\/jipb.70375","archive":["Portico"],"relation":{},"ISSN":["1672-9072","1744-7909"],"issn-type":[{"value":"1672-9072","type":"print"},{"value":"1744-7909","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]},"assertion":[{"value":"2026-05-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}