{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:05:01Z","timestamp":1789563901275,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Ploidy manipulation is a crucial strategy for generating germplasm in crop breeding. However, artificial genomic duplication, often induced by colchicine treatment, is associated with toxicity and unpredictability. Although mutations in\n                    <jats:italic>OSD1<\/jats:italic>\n                    have shown promise for inducing genomic duplication, the instability of ploidy across generations limits their practical application. In this study, we developed a Plant Polyploidization via Gene Interference (PPGI) system that utilizes transient RNAi\u2010mediated suppression of\n                    <jats:italic>OSD1<\/jats:italic>\n                    to efficiently induce artificial genomic duplication, demonstrating obvious potential for producing autotetraploids. We first validated this system by successfully generating PPGI\u2010induced autotetraploid plants from the Taichung65 cultivar. These PPGI\u2010induced plants exhibited notable differences from Taichung65 but resembled the existing Taichung65\u20104x line obtained through colchicine treatment. Haplotype analysis indicated that the\n                    <jats:italic>OSD1<\/jats:italic>\n                    RNAi fragment is conserved across 2,908 rice cultivars. Consequently, we employed the same PPGI vector to develop autotetraploid lines from various germplasms, including another\n                    <jats:italic>japonica<\/jats:italic>\n                    cultivar, seven\n                    <jats:italic>indica<\/jats:italic>\n                    cultivars, and one\n                    <jats:italic>Oryza rufipogon<\/jats:italic>\n                    line. The probability of genomic duplication achieved by our PPGI method was higher than that obtained by colchicine treatment. Typically, autotetraploid lines exhibit severe sterility in the first generation following polyploidization. Leveraging fertile neo\u2010tetraploid rice and the PPGI system, we designed and verified two strategies to directly induce fertile autotetraploid germplasms in the first generation, thereby substantially shortening the breeding cycle. Our method provides a universal, efficient, and non\u2010toxic approach for inducing autotetraploid rice germplasms and contributes to enriching fertile autotetraploid rice germplasm resources.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70393","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T12:17:17Z","timestamp":1789561037000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A robust biotechnology induces artificial genomic duplication via transient RNAi\u2010mediated suppression of\n                    <i>OSD1<\/i>\n                    in rice"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2959-7712","authenticated-orcid":false,"given":"Zijun","family":"Lu","sequence":"first","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3415-5396","authenticated-orcid":false,"given":"Lianjun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6517-9490","authenticated-orcid":false,"given":"Guobin","family":"Liang","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1637-1094","authenticated-orcid":false,"given":"Rou","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7647-1040","authenticated-orcid":false,"given":"Jia","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1920-1864","authenticated-orcid":false,"given":"Liang","family":"Fu","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0277-6864","authenticated-orcid":false,"given":"Xiangxing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture South China Agricultural University Guangzhou 510642 China"},{"name":"Key Laboratory for Enhancing Resource Use Efficiency of Crops in South China Ministry of Agriculture and Rural Affairs Guangzhou 510642 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