{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:59:30Z","timestamp":1790773170743,"version":"4.1.0"},"reference-count":61,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Excessive application of nitrogen (N) fertilizers, particularly nitrate (NO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    ), in protected agricultural systems has led to secondary soil salinization, posing a global threat to crop yield and quality. The regulatory network underlying tolerance to excess nitrate in tomato (\n                    <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    ) remains largely unexplored. Here, we report that\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosoglutathione reductase (GSNOR) positively regulates nitrate tolerance by reducing nitric oxide (NO) and\n                    <jats:italic>S<\/jats:italic>\n                    \u2011nitrosothiol (SNO) accumulation. We then identified the transcription factor SlMYB86 as a direct upstream activator of\n                    <jats:italic>SlGSNOR<\/jats:italic>\n                    , and further found that nitrate\u2011induced NO triggers\n                    <jats:italic>S<\/jats:italic>\n                    \u2011nitrosylation of SlMYB86 at Cys68 and Cys263. This post\u2011translational modification enhances SlMYB86 protein stability by blocking 26S proteasome\u2011mediated degradation, while also increasing its DNA\u2011binding affinity and the transcriptional activation of\n                    <jats:italic>SlGSNOR<\/jats:italic>\n                    , thereby enhancing nitrate tolerance. Moreover, Growth\u2010regulating factor 4 (SlGRF4) functions as an upstream regulator of this pathway, activating\n                    <jats:italic>SlMYB86<\/jats:italic>\n                    expression under nitrate stress and thereby enhancing tomato tolerance to excessive nitrate stress. Collectively, these findings establish a SlGRF4\u2013SlMYB86\u2013SlGSNOR module in which SlMYB86\n                    <jats:italic>S<\/jats:italic>\n                    \u2011nitrosylation establishes a negative feedback loop that couples NO signaling to NO clearance, maintaining redox homeostasis and promoting nitrate tolerance in tomato.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70408","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:21:58Z","timestamp":1790770918000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>S<\/i>\n                    \u2010nitrosylation of SlMYB86 within a SlGRF4\u2013SlMYB86\u2013SlGSNOR module enhances nitrate tolerance in tomato"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1005-3941","authenticated-orcid":false,"given":"Senlin","family":"Zeng","sequence":"first","affiliation":[{"name":"Faculty of Life 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