{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:51:42Z","timestamp":1791982302454,"version":"4.4.0"},"reference-count":76,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32573040"],"award-info":[{"award-number":["32573040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["JIPB"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Radish taproot thickening is primarily driven by vascular cambium (VC) activity. However, the transcriptional regulatory mechanisms underlying taproot thickening at a single\u2010cell resolution remain unclear. In this study, single\u2010nucleus isolation coupled with fluorescence\u2010activated cell sorting was used to construct a cell atlas of radish taproots using microdroplet\u2010based single\u2010nucleus RNA sequencing. Through canonical marker gene profiling and validation using laser capture microdissection transcriptome data, 12 cell types were identified, and the first single\u2010nucleus transcriptomic atlas was constructed for radish taproots. VC cells exhibited heterogeneous transcriptional states with varying levels of expression of cell cycle\u2010associated genes. Pseudotime analysis inferred a transcriptional trajectory from VC cells toward xylem parenchyma cells in radish taproots. Integrating pseudotime analysis with predicted protein\u2013protein interaction networks highlighted HOMEOBOX 8 (RsHB8) as a candidate regulator of the inferred VC\u2013to\u2013xylem parenchyma (XP) trajectory.\n                    <jats:italic>RsHB8m<\/jats:italic>\n                    overexpression increased taproot width and promoted xylem differentiation, whereas\n                    <jats:italic>RsHB8<\/jats:italic>\n                    \u2010silenced plants exhibited a reduced taproot width and narrower xylem region.\n                    <jats:italic>Xyloglucan endotransglucosylase\/hydrolase 22<\/jats:italic>\n                    (\n                    <jats:italic>RsXTH22<\/jats:italic>\n                    ) was identified as a target gene of RsHB8. LOB domain\u2010containing protein 21 (RsLBD21) interacted with RsHB8, inhibited its transcriptional activity, and attenuated the RsHB8\u2010mediated transcriptional activation of\n                    <jats:italic>RsXTH22<\/jats:italic>\n                    . Silencing\n                    <jats:italic>RsLBD21<\/jats:italic>\n                    significantly increased taproot and xylem width, whereas silencing\n                    <jats:italic>RsXTH22<\/jats:italic>\n                    resulted in the opposite phenotypes. Together, these findings establish a single\u2010nucleus transcriptomic atlas of radish taproot development and provide insights into the transcriptional regulation of VC differentiation during taproot thickening.\n                  <\/jats:p>","DOI":"10.1111\/jipb.70417","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T12:19:48Z","timestamp":1791980388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010nucleus RNA sequencing reveals a key regulator RsHB8 affecting vascular cambium activity and xylem differentiation during radish taproot thickening"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-7294-2227","authenticated-orcid":false,"given":"Feng","family":"Cui","sequence":"first","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"},{"name":"College of Horticulture and Landscape Architecture Yangzhou University Yangzhou 225009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0055-8272","authenticated-orcid":false,"given":"Jingxue","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8477-152X","authenticated-orcid":false,"given":"Hongyu","family":"Bi","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9508-0655","authenticated-orcid":false,"given":"Sichen","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4504-4108","authenticated-orcid":false,"given":"Xiaoqi","family":"Yuan","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8201-4743","authenticated-orcid":false,"given":"Boya","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1865-8041","authenticated-orcid":false,"given":"Liang","family":"Xu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-9190","authenticated-orcid":false,"given":"Yinbo","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture Yangzhou University Yangzhou 225009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6284-1675","authenticated-orcid":false,"given":"Xiaoli","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5671-1992","authenticated-orcid":false,"given":"Sen","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3834-834X","authenticated-orcid":false,"given":"Yuting","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0159-972X","authenticated-orcid":false,"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7355-4408","authenticated-orcid":false,"given":"Liwang","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetics &amp; Germplasm Enhancement and Utilization, College of Horticulture Nanjing Agricultural University Nanjing 211800 China"},{"name":"College of Horticulture and Landscape Architecture Yangzhou University Yangzhou 225009 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"4171","DOI":"10.1242\/dev.121.12.4171","article-title":"The expression of the Athb\u20108 homeobox gene is restricted to provascular cells in Arabidopsis thaliana","volume":"121","author":"Baima S.","year":"1995","journal-title":"Development"},{"key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1104\/pp.126.2.643","article-title":"The Arabidopsis ATHB\u20108 HD\u2010zip protein acts as a differentiation\u2010promoting transcription factor of the vascular meristems","volume":"126","author":"Baima S.","year":"2001","journal-title":"Plant Physiol."},{"key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"1956","DOI":"10.1126\/science.1090022","article-title":"A gene expression map of the Arabidopsis root","volume":"302","author":"Birnbaum K.","year":"2003","journal-title":"Science"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1038\/nature02100","article-title":"APL regulates vascular tissue identity in Arabidopsis","volume":"426","author":"Bonke M.","year":"2003","journal-title":"Nature"},{"key":"e_1_2_9_6_1","doi-asserted-by":"crossref","first-page":"2501","DOI":"10.1016\/j.cub.2019.06.037","article-title":"A cellular Insulator against CLE45 peptide signaling","volume":"29","author":"Breda A.S.","year":"2019","journal-title":"Curr. 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