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The actin cytoskeleton represents a highly dynamic filamentous system providing cell structure and mechanical forces to drive membrane protrusion, cell migration and vesicle trafficking.<\/jats:p><jats:p>Here, we apply the structured\u2010illumination microscopy (SIM) technique to analyse the actin cytoskeleton in fixed <jats:italic>Drosophila<\/jats:italic> Schneider (S2R+) cells, both in wild type and in cells depleted for WAVE, a major activator of Arp2\/3 mediated actin polymerization. In addition, we demonstrate that live cell SIM imaging also allows the visualization of actin\u2010driven lamellipodial membrane dynamics at high spatial resolution in S2R+ cells. Three dimensional (3D) SIM images of up to 70 \u03bcm thick <jats:italic>Drosophila<\/jats:italic> wild\u2010type and <jats:italic>abi\u2010<\/jats:italic>mutant egg chambers further enables us to resolve changes of actin structures in a multicellular context with an impressive lateral and axial resolution, which is not possible with conventional confocal microscopy. Thus, the combination of superresolution 3D microscopy with <jats:italic>Drosophila<\/jats:italic> genetics and cell biology allows detailed insights into the structural and molecular requirements of different actin\u2010dependent processes.<\/jats:p>","DOI":"10.1111\/jmi.12020","type":"journal-article","created":{"date-parts":[[2013,2,14]],"date-time":"2013-02-14T15:31:59Z","timestamp":1360855919000},"page":"224-231","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["A high resolution view of the fly actin cytoskeleton lacking a functional WAVE complex"],"prefix":"10.1111","volume":"251","author":[{"given":"T.","family":"ZOBEL","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Neurobiologie Universit\u00e4t M\u00fcnster  M\u00fcnster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"BOGDAN","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Neurobiologie Universit\u00e4t M\u00fcnster  M\u00fcnster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,2,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(70)90646-4"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(71)90420-4"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/35060104"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2008.09.011"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00663"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1305"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2867"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2008.12.001"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.200900123"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.02.003"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1119262109"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.07.058"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.120345"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(10)83010-1"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2011.08.011"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137395"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200109065"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.36.052802.114101"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2003.10.005"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.6.2268"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.10.024"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.090027"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2006.11.022"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060408-092949"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.110"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.11.6181"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1125"},{"key":"e_1_2_5_29_1","first-page":"1","article-title":"Cellular motility powered by actin filament assembly and disassembly","volume":"98","author":"Pollard T. 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