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On the other hand, we examine X\u2010ray phase\u2010contrast micro\u2010computed tomography (micro\u2010CT) using synchrotron radiation. Cryo\u2010imaging is a technique in which an electron multiplying charge coupled camera takes images of a cryo\u2010frozen specimen during the sectioning process. Subsequent image alignment and virtual stacking result in volumetric data. X\u2010ray phase\u2010contrast imaging is based on the minute refraction of X\u2010rays inside the specimen and features higher soft\u2010tissue contrast than conventional, attenuation\u2010based micro\u2010CT. To explore the potential of both techniques for studying whole mouse disease models, one mouse specimen was imaged using both techniques. Obtained data are compared visually and quantitatively, specifically with regard to the visibility of fine anatomical details. Internal structure of the mouse specimen is visible in great detail with both techniques and the study shows in particular that soft\u2010tissue contrast is strongly enhanced in the X\u2010ray phase images compared to the attenuation\u2010based images. This identifies phase\u2010contrast micro\u2010CT as a powerful tool for the study of small animal disease models.<\/jats:p>","DOI":"10.1111\/jmi.12094","type":"journal-article","created":{"date-parts":[[2013,11,15]],"date-time":"2013-11-15T21:07:23Z","timestamp":1384549643000},"page":"24-30","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Three\u2010dimensional imaging of whole mouse models: comparing nondestructive X\u2010ray phase\u2010contrast micro\u2010CT with cryotome\u2010based planar epi\u2010illumination imaging"],"prefix":"10.1111","volume":"253","author":[{"given":"A.","family":"TAPFER","sequence":"first","affiliation":[{"name":"Department of Physics and Institute of Medical Engineering Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"BECH","sequence":"additional","affiliation":[{"name":"Department of Physics and Institute of Medical Engineering Technische Universit\u00e4t M\u00fcnchen  Munich Germany"},{"name":"Medical Radiation Physics Clinical Sciences Lund University  Lund Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"ZANETTE","sequence":"additional","affiliation":[{"name":"Department of Physics and Institute of Medical Engineering Technische Universit\u00e4t M\u00fcnchen  Munich Germany"},{"name":"European Synchrotron Radiation Facility  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"SYMVOULIDIS","sequence":"additional","affiliation":[{"name":"Institute for Biological and Medical Imaging Helmholtz Zentrum M\u00fcnchen  Neuherberg Germany"},{"name":"Chair for Biological Imaging Technische Universit\u00e4t M\u00fcnchen  Munich Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"STANGL","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology 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