{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T23:23:35Z","timestamp":1791069815745,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T00:00:00Z","timestamp":1404777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Deutsche Forschungsgesellschaft","award":["SPP1420"],"award-info":[{"award-number":["SPP1420"]}]},{"name":"Deutsche Forschungsgesellschaft","award":["Ra1380\/7"],"award-info":[{"award-number":["Ra1380\/7"]}]},{"name":"Deutsche Forschungsgesellschaft","award":["Ge1894\/3"],"award-info":[{"award-number":["Ge1894\/3"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The architectural properties of the osteocyte cell network provide a valuable basis for understanding the mechanisms of bone remodelling, mineral homeostasis, ageing and pathologies. Recent advances in synchrotron microtomography enable unprecedented three\u2010dimensional imaging of both the bone lacunar network and the extracellular matrix. Here, we investigate the three\u2010dimensional morphological properties of osteocyte lacunae in human healthy and bisphosphonate\u2010related osteonecrotic jaw bone based on synchrotron X\u2010ray computed tomography images, with a spatial isotropic voxel size of 300 nm. Bisphosphonate\u2010related osteonecrosis of the jaw is a relatively new disease with increasing incidence, which remains poorly understood. A step forward in elucidating this malady is to assess whether, and how, the morphology of the osteocyte lacunar network is modified in the affected jaw tissue. We evaluate thousands of cell lacunae from five specimens of which three originate from patients diagnosed with bisphosphonate\u2010associated osteonecrosis. In this exploratory study, we report three\u2010dimensional quantitative results on lacunar volumes (296\u2013502 \u03bcm<jats:sup>3<\/jats:sup>), shape (approximated by an ellipsoidal shape with principal axes <jats:italic>a &gt; b &gt; c<\/jats:italic>, such that <jats:italic>a = 2.2b<\/jats:italic> and <jats:italic>a = 4c<\/jats:italic>) and spatial distribution (i.e., 50% of the mineralized matrix volume is located within 12 \u03bcm to the closest lacunar boundary) at submicron resolution on such specimens. We observe that the average lacunar volumes of the bisphosphonate\u2010related osteonecrotic jaw specimens were within the range of volumes found in the two specimens originating from healthy donors and conclude that lacunar volumes are not the key element in the course of bisphosphonate\u2010related osteonecrotic jaw. In three out of five specimens we observe lacunar volume sizes in segmented osteons to be significantly different compared to lacunar volumes in the adjacent tissue regions. Furthermore, we quantify the number of lacunae containing small dense objects (on average 9%). In contrast to lacunar morphology we report the lacunar density (16\u00a0000\u201350\u00a0000 per mm<jats:sup>3<\/jats:sup>) to be different in jaw bone tissue compared to what has been reported in femoral sites.<\/jats:p>","DOI":"10.1111\/jmi.12147","type":"journal-article","created":{"date-parts":[[2014,7,8]],"date-time":"2014-07-08T13:57:22Z","timestamp":1404827842000},"page":"158-168","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Accessing osteocyte lacunar geometrical properties in human jaw bone on the submicron length scale using synchrotron radiation \u03bcCT"],"prefix":"10.1111","volume":"255","author":[{"given":"BERNHARD","family":"HESSE","sequence":"first","affiliation":[{"name":"Berlin\u2010Brandenburg School for Regenerative Therapies and Julius Wolff Institut Charit\u00e9\u2010Universit\u00e4tsmedizin Berlin  Berlin Germany"},{"name":"European Synchrotron Radiation Facility  Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"NILS","family":"M\u00c4NNICKE","sequence":"additional","affiliation":[{"name":"Berlin\u2010Brandenburg School for Regenerative Therapies and Julius Wolff Institut Charit\u00e9\u2010Universit\u00e4tsmedizin Berlin  Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ALEXANDRA","family":"PACUREANU","sequence":"additional","affiliation":[{"name":"European Synchrotron Radiation Facility  Grenoble France"},{"name":"Universit\u00e9 de Lyon, CREATIS CNRS UMR 5220 Inserm U1044 INSA\u2010Lyon Universit\u00e9 Lyon 1  Lyon France"},{"name":"Centre for Image Analysis and Science for Life Laboratory Uppsala University  Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PETER","family":"VARGA","sequence":"additional","affiliation":[{"name":"Berlin\u2010Brandenburg School for Regenerative Therapies and Julius Wolff Institut Charit\u00e9\u2010Universit\u00e4tsmedizin Berlin  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