{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:05:36Z","timestamp":1792670736242,"version":"4.5.0"},"reference-count":11,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T00:00:00Z","timestamp":1454889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Transmission electron microscopy (TEM) provides sub\u2010nanometre\u2010scale details in volumetric samples. Samples such as pathology tissue specimens are often stained with a metal element to enhance contrast, which makes them opaque to optical microscopes. As a result, it can be a lengthy procedure to find the region of interest inside a sample through sectioning. We describe micro\u2010CT scouting for TEM that allows noninvasive identification of regions of interest within a block sample to guide the sectioning step. In a tissue pathology study, a bench\u2010top micro\u2010CT scanner with 10 \u03bcm resolution was used to determine the location of patches of the mucous membrane in osmium\u2010stained human nasal scraping samples. Once the regions of interest were located, the sample block was sectioned to expose that location, followed by ultra\u2010thin sectioning and TEM to inspect the internal structure of the cilia of the membrane epithelial cells with nanometre resolution. This method substantially reduced the time and labour of the search process from typically 20 sections for light microscopy to three sections with no added sample preparation.<\/jats:p>","DOI":"10.1111\/jmi.12385","type":"journal-article","created":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T12:14:19Z","timestamp":1454933659000},"page":"113-117","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Micro\u2010CT scouting for transmission electron microscopy of human tissue specimens"],"prefix":"10.1111","volume":"263","author":[{"given":"A.G.","family":"MORALES","sequence":"first","affiliation":[{"name":"National Heart Lung and Blood Institute National Institutes of Health  Bethesda Maryland U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.S.","family":"STEMPINSKI","sequence":"additional","affiliation":[{"name":"National Heart Lung and Blood Institute National Institutes of Health  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