{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,17]],"date-time":"2026-05-17T04:14:18Z","timestamp":1778991258254,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Laser ablation machining or microtomy (LAM) is a relatively new approach to producing slide mounted sections of translucent materials. We evaluated the method with a variety of problems from the bone, joint and dental tissues fields where we require thin undecalcified and undistorted sections for correlative light microscopy (LM) and backscattered electron scanning electron microscopy (BSE SEM). All samples were embedded in poly\u2010methylmethacrlate (PMMA) and flat block surfaces had been previously studied by BSE\u2010SEM and confocal scanning light microscopy (CSLM). Most were also studied by X\u2010yay microtomography (XMT). The block surface is stuck to a glass slide with cyanoacrylate adhesive. Setting the section thickness and levelling uses inbuilt optical coherence tomographic imaging. Tight focusing of near\u2010infrared laser radiation in the sectioning plane gives extreme intensities causing photodisruption of material at the focal point. The laser beam is moved by a fast scanner to write a cutting line, which is simultaneously moved by an <jats:italic>XY<\/jats:italic> positioning unit to create a sectioning plane. The block is thereby released from the slide, leaving the section stuck to the slide. Light, wet polishing on the finest grade (4000 grit) silicon carbide polishing paper is used to remove a 1\u20132 \u03bcm thick damaged layer at the surface of the section. Sections produced by laser cutting are fine in quality and superior to those produced by mechanical cutting and can be thinner than the \u2018voxel\u2019 in most laboratory X\u2010ray microtomography systems. The present extensive pilot studies have shown that it works to produce samples which we can study by both light and electron microscopy.<\/jats:p>","DOI":"10.1111\/jmi.12689","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T12:14:53Z","timestamp":1519733693000},"page":"17-30","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of laser ablation microtomy for correlative microscopy of hard tissues"],"prefix":"10.1111","volume":"271","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9871-5498","authenticated-orcid":false,"given":"A.","family":"BOYDE","sequence":"first","affiliation":[{"name":"Dental Physical Sciences Queen Mary University of London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2011-1814"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002858"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C6MT00073H"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-83496-7_6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-7580.2003.00213.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v024a11"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12226"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.22435"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v021a35"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/joa.12628"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v009a05"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900600"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/sca.20139"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1302\/0301-620X.77B5.7559718"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1982.tb00172.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215549704500215"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.10282"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520297709116778"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01605129"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jmi.12457"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211396"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2012.02.004"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms16059600"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v027a16"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/opph.201190252"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/evj.12036"},{"key":"e_1_2_7_28_1","first-page":"35","volume-title":"Techniques of Biocompatibility Testing","author":"Plenk H. 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