{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T10:52:13Z","timestamp":1752231133019,"version":"3.37.3"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T00:00:00Z","timestamp":1526342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31372231"],"award-info":[{"award-number":["31372231"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The endeavours to reveal the tracheal system of insects and some arachnids has a long history. The traditional way to observe a tracheal system in an insect body is by utilising the glycerin immersion method. In this study, we developed the lactic acid immersion method, which reveals a more complete tracheal system. By mounting various types of live specimens or body parts directly into lactic acid, multiple intact and complex tracheal systems were clearly visualised. The lactic acid immersion contributed to revealing tracheal systems by penetrating body tissue while reserving enough time for observation before the penetration of the tracheae. Preliminary comparisons were conducted between lactic acid and other mediae, including glycerin. It turned out that lactic acid immersion provides better details and more distinct structures. In our test, the optimal time for observing the tracheal system was 10\u201325 min after the organism was immersed in lactic acid.<\/jats:p>","DOI":"10.1111\/jmi.12711","type":"journal-article","created":{"date-parts":[[2018,5,15]],"date-time":"2018-05-15T14:35:29Z","timestamp":1526394929000},"page":"230-236","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Visualisation of insect tracheal systems by lactic acid immersion"],"prefix":"10.1111","volume":"271","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5025-5592","authenticated-orcid":false,"given":"Y.","family":"RUAN","sequence":"first","affiliation":[{"name":"School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic  Shenzhen Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"LI","sequence":"additional","affiliation":[{"name":"School of Forest Resources and Conservation University of Florida  Gainesville Florida U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"ZHANG","sequence":"additional","affiliation":[{"name":"School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic  Shenzhen Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"X.","family":"CHEN","sequence":"additional","affiliation":[{"name":"School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic  Shenzhen Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"LIU","sequence":"additional","affiliation":[{"name":"School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic  Shenzhen Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"WANG","sequence":"additional","affiliation":[{"name":"Shanghai Entry\u2010Exit Inspection and Quarantine Bureau  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"JIANG","sequence":"additional","affiliation":[{"name":"School of Applied Chemistry and Biological Technology, Postdoctoral Innovation Practice Base, Shenzhen Polytechnic  Shenzhen Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.014498"},{"key":"e_1_2_7_3_1","first-page":"481","article-title":"Respiration system of cockroaches. 2. 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