{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:19:40Z","timestamp":1784168380818,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000046","name":"National Research Council Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000046","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008920","name":"University of Saskatchewan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008920","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:label\/><jats:p>The incudostapedial joint (ISJ) of the middle ear is important for proper transmission of sound energy to the cochlea. Recently, the biomechanics of the ISJ have been investigated using finite\u2010element (FE) modelling, using simplified geometry. The objective of the present study was to investigate the feasibility of synchrotron\u2010radiation phase\u2010contrast imaging (SR\u2010PCI) in visualising the ISJ ultrastructure. Three human cadaveric ISJs were dissected and scanned using SR\u2010PCI at 0.9 \u00b5m isotropic voxel size. One of the samples was previously scanned at 9 \u00b5m voxel size. The images were visually compared and contrast\u2010to\u2010noise ratios (CNRs) were calculated (of both bone and soft tissues) for quantitative comparisons. The ISJ ultrastructure as well as adjacent bone and soft tissues were clearly visible in images with a 0.9 \u00b5m voxel size. The CNRs of the 0.9 \u00b5m images were relatively lower than those of the 9 \u00b5m scans, while the ratio of bone to soft tissue CNRs were higher, indicating better discernibility of bone from soft tissue in the 0.9 \u00b5m scans. This study was the first known attempt to image the ISJ ultrastructure using an SR\u2010PCI scanner at submicron voxel size and results suggest that this method was successful. Future studies are needed to optimise the contrast and test the feasibility of imaging the ISJ <jats:italic>in situ<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Lay Description<\/jats:title><jats:p>The human middle ear consists of the eardrum, three small bones (the malleus, incus and stapes) and two joints connecting the bones (the incudostapedial joint and the incudomallear joint). The role of the middle ear is to amplify and transfer sound energy to the cochlea, the end organ of hearing. The incudostapedial joint (ISJ) of the middle ear is a synovial joint which is important for proper transmission of sound energy to the cochlea. Similar to other synovial joints it consists of meniscus, fluid and articulating surfaces. Recently, the biomechanics of the ISJ have been investigated using computational models, using grossly simplified geometry. Synchrotron radiation phase contrast imaging (SR\u2010PCI) is a high\u2010resolution imaging technique used to visualise small structures in three dimensions. The objective of the present study was to investigate the feasibility of using SR\u2010PCI in visualising the ISJ ultrastructure. Three human cadaveric ISJs were dissected and scanned using SR\u2010PCI at 0.9 \u00b5m isotropic voxel size. One of the samples was previously scanned at 9 \u00b5m voxel size. The images were both qualitatively and quantitatively compared. This study was the first known attempt to image the ISJ ultrastructure using an SR\u2010PCI scanner at submicron voxel size and results suggest that this method was successful. Future studies are needed to optimise the contrast and feasibility of imaging the ISJ in situ.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmi.12864","type":"journal-article","created":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T07:06:45Z","timestamp":1580195205000},"page":"61-70","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["High\u2010resolution imaging of the human incudostapedial joint using synchrotron\u2010radiation phase\u2010contrast imaging"],"prefix":"10.1111","volume":"277","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5467-4276","authenticated-orcid":false,"given":"S.A.","family":"ROHANI","sequence":"first","affiliation":[{"name":"Department of Otolaryngology \u2013 Head and Neck Surgery Western University  London Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"ALLEN","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Western University  London Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"GARE","sequence":"additional","affiliation":[{"name":"Department of Electrical and Computer Engineering Western University  London Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"ZHU","sequence":"additional","affiliation":[{"name":"Bio\u2010Medical Imaging and Therapy Facility, Canadian Light Source Inc. 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