{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T04:02:44Z","timestamp":1790654564802,"version":"4.1.0"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,29]],"date-time":"2022-04-29T00:00:00Z","timestamp":1651190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The present research comes up with a novel DNA\u2010loaded poly\u2010L\u2010lysine (PLL)\/hyaluronan (HA) nanocarrier (DNA\u2010loaded PLL\/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA\u2010loaded PLL molecules by HA. Fourier\u2010transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission\u2010scanning electron microscopy\u00a0(FE\u2010SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of \u201324 \u00b1 3\u00a0mV, with an average size of 138 \u00b1 6 nm, in an ellipsoid\u2010shape with smooth surfaces. The DNA loading efficiency (LE) measured by DNA absorbance was around 95 %. The MTT assay showed that the developed NCs are non\u2010toxic to the cells. Furthermore, the uptake of the DNA\u2010loaded PLL\/HA NCs by the human embryonic kidney (HEK)\u2010293T cells was evaluated by a flow cytometry method, and demonstrated high potential cellular uptake over 90% for transferring the gene to HEK\u2010293T cells at the optimised conditions. Therefore, the DNA\u2010loaded PLL\/HA NCs are the potent strategy for developing nanosystems for gene delivery applications.<\/jats:p>","DOI":"10.1111\/jmi.13107","type":"journal-article","created":{"date-parts":[[2022,4,20]],"date-time":"2022-04-20T19:59:15Z","timestamp":1650484755000},"page":"32-44","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Poly\u2010L\u2010lysine\/hyaluronan nanocarriers as a novel nanosystem for gene delivery"],"prefix":"10.1111","volume":"287","author":[{"given":"Masoumeh","family":"Souri","sequence":"first","affiliation":[{"name":"Cellular and Molecular Research Center Iran University of Medical Sciences  Tehran Iran"},{"name":"Faculty of Advanced Technologies in Medicine, Department of Medical Nanotechnology Iran University of Medical Sciences  Tehran Iran"},{"name":"Advances Nanobiotechnology and Nanomedicine Research Group (ANNRG) Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Aref","family":"Bagherzadeh","sequence":"additional","affiliation":[{"name":"Student Research Committee Jahrom University of Medical Sciences  Jahrom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7109-4476","authenticated-orcid":false,"given":"Mirza Ali","family":"Mofazzal Jahromi","sequence":"additional","affiliation":[{"name":"Research Center for Noncommunicable Diseases Jahrom University of Medical Sciences  Jahrom Iran"},{"name":"Department of Immunology School of Medicine Jahrom University of Medical Sciences  Jahrom Iran"},{"name":"Department of Advanced Medical Sciences &amp; Technologies School of Medicine Jahrom University of Medical Sciences  Jahrom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Mohammad\u2010Beigi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Abdoli","sequence":"additional","affiliation":[{"name":"Department of Parasitology, and Mycology, School of Medicine Jahrom University of Medical Sciences  Jahrom Iran"},{"name":"Zoonoses Research Center Jahrom University of Medical Sciences  Jahrom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamed","family":"Mir","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Nutrition, School of Medicine Jahrom University of Medical Sciences  Jahrom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abazar","family":"Roustazadeh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Nutrition, School of Medicine Jahrom University of Medical Sciences  Jahrom Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majid","family":"Pirestani","sequence":"additional","affiliation":[{"name":"Faculty of Medical Sciences Parasitology and Entomology Department Tarbiat Modares University  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parham","family":"Sahandi Zangabad","sequence":"additional","affiliation":[{"name":"Advances Nanobiotechnology and Nanomedicine Research Group (ANNRG) Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jafar","family":"Kiani","sequence":"additional","affiliation":[{"name":"Faculty of Advanced Technologies in Medicine Department of Molecular Medicine Iran University of Medical Sciences  Tehran Iran"},{"name":"Oncopathology Research Center Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amirmahmoud","family":"Bakhshayesh","sequence":"additional","affiliation":[{"name":"Department of Physics Sharif University of Technology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Jahani","sequence":"additional","affiliation":[{"name":"Department of Physics Sharif University of Technology  Tehran Iran"},{"name":"Department of Chemistry Sharif University of Technology  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Taghi","family":"Joghataei","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center Iran University of Medical Sciences  Tehran Iran"},{"name":"Faculty of Medicine Department of Anatomy Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Karimi","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center Iran University of Medical Sciences  Tehran Iran"},{"name":"Faculty of Advanced Technologies in Medicine, Department of Medical Nanotechnology Iran University of Medical Sciences  Tehran Iran"},{"name":"Advances Nanobiotechnology and Nanomedicine Research Group (ANNRG) Iran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.6b01774"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1007\/978-1-59745-561-9_1","article-title":"The development of gene therapy: From monogenic recessive disorders to complex diseases such as cancer","volume":"542","author":"Gillet J.\u2010P.","year":"2009","journal-title":"Gene Therapy of Cancer: Methods and Protocols"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2222-1808(14)60470-9"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(01)00494-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.matsci.31.1.25"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-003-1321-8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/71889"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3763"},{"key":"e_1_2_8_10_1","unstructured":"Mahato R. I. Han S.\u2010O. &Furgeson D. Y.(2004).Cationic lipopolymer as biocompatible gene delivery agent. Google Patents."},{"key":"e_1_2_8_11_1","volume-title":"Gene delivery to mammalian cells","author":"Heiser W. C.","year":"2004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2016.1178131"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.17.3694"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/btm2.10019"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics11070338"},{"issue":"1","key":"e_1_2_8_16_1","first-page":"134","article-title":"Evaluation of antibacterial effect of curcumin loaded chitosan nanoparticles","volume":"5","author":"Mofazzal Jahromi M. A.","year":"2015","journal-title":"Journal of Advanced Biomedical Sciences"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-009-9140-1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1775"},{"key":"e_1_2_8_19_1","first-page":"191","volume-title":"Cationic liposome\u2013nucleic acid complexes for gene delivery and silencing: Pathways and mechanisms for plasmid DNA and siRNA. Nucleic acid transfection","author":"Ewert K. K.","year":"2010"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955710791185109"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jddst.2020.102118"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.201200303"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4781(98)00274-7"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc050264g"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0169-409X(97)00110-5"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(02)00002-0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/bm500843r"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5NR07004J"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms12021371"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.6.3793"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2012.02.021"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b01611"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.252"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13205-016-0379-9"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2012.11.009"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.01.030"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.04.030"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.6b00240"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.07.015"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.7.13.7691670"},{"key":"e_1_2_8_41_1","volume-title":"Zeta potential in colloid science: Principles and applications","author":"Hunter R. J.","year":"2013"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.15171\/ijb.1012"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2016.03.036"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18061291"},{"issue":"1","key":"e_1_2_8_45_1","first-page":"27","article-title":"Evaluation of physiochemical properties of curcumin\u2010loaded chitosan tripolyphosphate nanoparticles","volume":"20","author":"Mofazzal Jahromi M. A.","year":"2016","journal-title":"Molecular Characterization of Parasitic Worm and Protozoa in Iran"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.252"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsnano.7b01385"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200900916"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.04.030"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4TB01071J"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-3155-12-5"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3330"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2012.06.079"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S146679"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201900716"},{"issue":"2","key":"e_1_2_8_56_1","first-page":"71","article-title":"In vitro labeling of neural stem cells with poly\u2010L\u2010lysine coated super paramagnetic nanoparticles for green fluorescent protein transfection","volume":"17","author":"Albukhaty S.","year":"2013","journal-title":"Iran Biomedical Journal"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/bc800206r"}],"container-title":["Journal of Microscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jmi.13107","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jmi.13107","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jmi.13107","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T00:41:22Z","timestamp":1692837682000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jmi.13107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,29]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/jmi.13107"],"URL":"https:\/\/doi.org\/10.1111\/jmi.13107","archive":["Portico"],"relation":{},"ISSN":["0022-2720","1365-2818"],"issn-type":[{"value":"0022-2720","type":"print"},{"value":"1365-2818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,29]]},"assertion":[{"value":"2022-01-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}