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The noise increases measurement uncertainty and complicates further image processing routines such as deconvolution, object detection and segmentation. State\u2010of\u2010the\u2010art denoisers are computationally expensive and require training using large datasets, which are not available in cases of typical biological imaging experiments with rather scarce and unlabelled data. Here, we show that a denoiser can be trained using a single image containing a cropped out object of interest, where we exploit the symmetry often present in biological structures at molecular scales. As only a single example is used during training, our method can be trained even with limited computational resources, obtaining competitive denoising performance compared to the state\u2010of\u2010the\u2010art\u00a0methods.<\/jats:p>","DOI":"10.1111\/jmi.70062","type":"journal-article","created":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T12:18:03Z","timestamp":1769170683000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["3SD: Rotational symmetry single\u2010shot denoising in fluorescence microscopy"],"prefix":"10.1111","author":[{"given":"Tijmen H.","family":"de Wolf","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics Erasmus University Medical Center Rotterdam the Netherlands"},{"name":"Department of Radiology &amp; Nuclear Medicine Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pleun","family":"Engbers","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Erasmus University Medical Center Rotterdam the Netherlands"},{"name":"Department of Radiology &amp; Nuclear Medicine Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justine","family":"Perrin","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Erasmus University Medical Center Rotterdam the Netherlands"},{"name":"Department of Radiology &amp; Nuclear Medicine Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie","family":"Nonnekens","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics Erasmus University Medical Center Rotterdam the Netherlands"},{"name":"Department of Radiology &amp; Nuclear Medicine Erasmus University Medical Center Rotterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ihor","family":"Smal","sequence":"additional","affiliation":[{"name":"Cell Biology, Neurobiology and Biophysics, Department of Biology, Faculty of Science Utrecht University Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,23]]},"reference":[{"key":"e_1_2_9_2_1","unstructured":"Miyawaki A. 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