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The entry requirement, however, of a transmission electron microscope (TEM) adapted with bespoke software for coordinated sample stage rotation and continuous electron diffraction data acquisition has generally inhibited the wider uptake of 3DED. To address this limitation, we present novel\n                    <jats:italic>DigitalMicrograph<\/jats:italic>\n                    script\n                    <jats:italic>GiveMeED<\/jats:italic>\n                    appropriate for controlled 3DED data acquisition. The collection of useable reflections beyond 0.8 \u00c5 makes 3DED crystallographic processing effectively routine, using standard software and workflows derived from single\u2010crystal X\u2010ray diffraction (SCXRD) techniques. A full experimental workflow for 3DED on a conventional TEM is described in practical terms, in combination with direct imaging, and energy dispersive X\u2010ray spectroscopy (EDS) and electron energy loss spectroscopy (EELS), for the return of comprehensive correlative descriptions of crystal morphologies and sample compositions, with due regard for the quantification of electron flux at each stage of the characterisation process. The accuracy and effectiveness of\n                    <jats:italic>GiveMeED<\/jats:italic>\n                    is demonstrated through structure solutions for case study paracetamol, copper(II) phthalocyanine, and percholorocoronene samples, characterised in their near\u2010native states under controlled low dose conditions at either room or cryogenic temperatures, with determined unit cell parameters and atomic connectivity matching accepted literature X\u2010ray structures for these compounds. To promote the wider adoption of 3DED, we make\n                    <jats:italic>GiveMeED<\/jats:italic>\n                    freely available for use and modification, in support of greater uptake and utilisation of structure solution procedures via electron diffraction.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>LAY DESCRIPTION<\/jats:bold>\n                    : Crystallography is the study of crystal structure and properties via diffraction pattern analysis, generated transmitting short\u2010wavelength radiation (X\u2010rays, electrons) through molecular crystals. A key application is solving crystal structures, in fields such as drug discovery, protein structure investigation, and framework material synthesis. Three\u2010dimensional electron diffraction (3DED) is a powerful technique for determining crystal structures, often performed using a transmission electron microscope (TEM), suited to analysis of sub\u2010micron sized crystals that are too small for X\u2010ray diffraction methods, providing crystallographic characterisation of materials otherwise be inaccessible to diffraction methods. In a 3DED experiment, a series of diffraction patterns are recorded from a crystal rotated under electron beam illumination while the microscope is in diffraction mode. The 2D diffraction patterns are used to reconstruct the 3D diffraction sphere, to which established crystallographic techniques are applied to solve the crystal structure. Correlative structural and chemical analysis is achieved by acquiring images and spectra from the same specimen volume. Broad adoption of 3DED has been limited by access to specialised knowledge and custom software needed to control the TEM during 3DED experiments, and existing software options may not be compatible with all instrument setups. In this work we present a novel\n                    <jats:italic>DigitalMicrograph<\/jats:italic>\n                    script,\n                    <jats:italic>GiveMeED<\/jats:italic>\n                    , designed to enable reproducible 3DED experiments with on compatible TEM systems without requiring extensive modifications or custom coding. We demonstrate the capabilities of\n                    <jats:italic>GiveMeED<\/jats:italic>\n                    by solving the structures of small molecules paracetamol, percholorocoronene, and copper(II)\u2010phthalocyanine. We describe a comprehensive experimental workflow combining 3DED with TEM imaging and spectroscopy, care given to the precise control and quantification of electron fluence at each stage of the experimental workflow in order to limit electron beam damage and maintain the specimen at near\u2010native state during the diffraction experiment.\n                    <jats:italic>GiveMeED<\/jats:italic>\n                    is available via GitHub for use and modification, enabling the microscopy community greater access to 3DED. In providing a robust, repeatable workflow based on open\u2010source software, this work advances the routine use of electron diffraction on standard TEM hardware.\n                  <\/jats:p>","DOI":"10.1111\/jmi.70151","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T19:08:26Z","timestamp":1784833706000},"page":"263-276","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Practical crystallography with a transmission electron microscope"],"prefix":"10.1111","volume":"303","author":[{"given":"Benjamin L","family":"Weare","sequence":"first","affiliation":[{"name":"Nanoscale and Microscale Research Centre University of Nottingham  Nottingham UK"},{"name":"School of Chemistry University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayleigh L Y","family":"Fung","sequence":"additional","affiliation":[{"name":"Department of Computer Science Nottingham Trent University  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Cardillo\u2010Zallo","sequence":"additional","affiliation":[{"name":"Nanoscale and Microscale Research Centre University of Nottingham  Nottingham UK"},{"name":"School of Pharmacy University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William J","family":"Cull","sequence":"additional","affiliation":[{"name":"School of Chemistry University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W","family":"Fay","sequence":"additional","affiliation":[{"name":"Nanoscale and Microscale Research Centre University of Nottingham  Nottingham UK"},{"name":"Department of Mechanical Materials &amp; Manufacturing Engineering Faculty of Engineering University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen 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