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Although SXT has been extensively used to study remodelling in more than 130 mammalian cell lines, its application to plants remains largely unexplored. In this study, we present the first approach at full\u2010rotation SXT imaging of spreading earthmoss\n                    <jats:italic>P. patens<\/jats:italic>\n                    , which is a key model organism for studying plant cell architecture and developmental processes. We show that high\u2010resolution, three\u2010dimensional imaging of fully hydrated\n                    <jats:italic>P. patens<\/jats:italic>\n                    protoplasts enable visualisation of organelle morphology and distribution. These proof\u2010of\u2010concept results demonstrate the potential of SXT to capture ultrastructural changes in plant cells, depending on developmental cues, stress responses, and genotype\u2010dependent\u00a0variation.\n                  <\/jats:p>","DOI":"10.1111\/jmi.70164","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:40:19Z","timestamp":1791384019000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Visualising\n                    <i>Physcomitrium patens<\/i>\n                    protoplasts with full\u2010rotation soft X\u2010ray tomography"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3773-7647","authenticated-orcid":false,"given":"Fariha Mahzabin","family":"Annesha","sequence":"first","affiliation":[{"name":"Centre for Organismal Studies (COS) Heidelberg University Heidelberg Germany"},{"name":"HGSMathComp Heidelberg University Heidelberg Germany"},{"name":"Institute for Microstructure Technology (IMT) Karlsruhe Institute of Technology Eggenstein\u2010Leopoldshafen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7444-6222","authenticated-orcid":false,"given":"Sadia S.","family":"Tamanna","sequence":"additional","affiliation":[{"name":"Department of Molecular Botany RPTU Kaiserslautern\u2010Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Grasser","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies (COS) Heidelberg University Heidelberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4061-1175","authenticated-orcid":false,"given":"Stefanie J.","family":"M\u00fcller\u2010Sch\u00fcssele","sequence":"additional","affiliation":[{"name":"Department of Molecular Botany RPTU Kaiserslautern\u2010Landau Kaiserslautern Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9774-3833","authenticated-orcid":false,"given":"Venera","family":"Weinhardt","sequence":"additional","affiliation":[{"name":"Centre for Organismal Studies (COS) Heidelberg University Heidelberg Germany"},{"name":"Institute for Microstructure Technology (IMT) Karlsruhe Institute of Technology Eggenstein\u2010Leopoldshafen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/ETLS20170086"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551\u2010019\u201000560\u2010z"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1533"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","unstructured":"Hua M. D.\u2010S. Lin Y.\u2010H. Hsieh C.\u2010C. Chen C.\u2010L. &Lin Z.\u2010J.(2025).Three dimensional visualization of subcellular and suborganellar structures in near\u2010native plant cells by synchrotron soft x\u2010ray tomography.Frontiers in Plant Science 16.https:\/\/doi.org\/10.3389\/fpls.2025.1705667","DOI":"10.3389\/fpls.2025.1705667"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201031024\u20101"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.111.191460"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2022.101176"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00828"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.049023"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1088\/2515\u20107647\/abfc5a"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010025\u201029385\u20105"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00294\u2010003\u20100458\u20104"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00392232"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/adpr.202200142"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201078150\u20103"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102229\u201012\u201070"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2015.00078"},{"key":"e_1_2_6_19_1","unstructured":"Dragonfly. 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