{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:17:17Z","timestamp":1788520637199,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008398","name":"Villum Fonden","doi-asserted-by":"publisher","award":["VIL50411"],"award-info":[{"award-number":["VIL50411"]}],"id":[{"id":"10.13039\/100008398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Extracellular matrix (ECM) micropatterns provide well\u2010defined adhesive environments for investigating how geometric confinement influences cell migration. Here, we combined ECM protein micropatterning with quantitative live\u2010cell microscopy to examine NIH 3T3 fibroblast migration on straight and sinusoidal adhesive tracks. Time\u2010lapse bright\u2010field imaging, actin fluorescence imaging, centroid tracking, migration\u2010rate quantification and aspect\u2010ratio analysis revealed distinct migration behaviours under confined adhesion. On straight tracks, cells exhibited either oscillatory migration, characterised by repeated cycles of protrusion, retraction, and migration\u2010rate bursts, or confined migration, characterised by limited centroid displacement and slow morphological remodelling. Sinusoidal tracks further introduced curvature\u2010associated changes in migration dynamics, with cells adjusting leading\u2010edge orientation along the curved path or becoming locally confined within curved regions. These observations demonstrate that adhesive geometry influences cell migration beyond simple path guidance and highlight the value of engineered ECM micropatterns combined with live\u2010cell microscopy for resolving dynamic migration behaviours under confined conditions.<\/jats:p>","DOI":"10.1111\/jmi.70166","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:39:46Z","timestamp":1788518386000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Geometry\u2010dependent migration dynamics of fibroblasts on ECM protein micropatterns"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1956-416X","authenticated-orcid":false,"given":"Yuge","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Chemistry Interdisciplinary Nanoscience Center (iNANO), Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Chemistry Interdisciplinary Nanoscience Center (iNANO), Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3004-5958","authenticated-orcid":false,"given":"Lasse","family":"Hyldgaard Klausen","sequence":"additional","affiliation":[{"name":"Department of Chemistry Interdisciplinary Nanoscience Center (iNANO), Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2025-2171","authenticated-orcid":false,"given":"Mingdong","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Chemistry Interdisciplinary Nanoscience Center (iNANO), Aarhus University  Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"1704","DOI":"10.1126\/science.1092053","article-title":"Cell migration: Integrating signals from front to back","volume":"302","author":"Ridley A. J.","year":"2003","journal-title":"Science"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/nrm2720","article-title":"Collective cell migration in morphogenesis, regeneration and cancer","volume":"10","author":"Friedl P.","year":"2009","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/s41580-021-00366-6","article-title":"The principles of directed cell migration","volume":"22","author":"SenGupta S.","year":"2021","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/s41580-023-00583-1","article-title":"Cell\u2013extracellular matrix mechanotransduction in 3D","volume":"24","author":"Saraswathibhatla A.","year":"2023","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1016\/j.tcb.2016.02.002","article-title":"Front\u2013rear polarization by mechanical cues: From single cells to tissues","volume":"26","author":"Ladoux B.","year":"2016","journal-title":"Trends in Cell Biology"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1038\/nrc.2016.123","article-title":"Cancer cell motility: Lessons from migration in confined spaces","volume":"17","author":"Paul C. D.","year":"2017","journal-title":"Nature Reviews Cancer"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1016\/j.cell.2015.01.056","article-title":"Actin flows mediate a universal coupling between cell speed and cell persistence","volume":"161","author":"Maiuri P.","year":"2015","journal-title":"Cell"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/S0092-8674(00)81280-5","article-title":"Cell migration: A physically integrated molecular process","volume":"84","author":"Lauffenburger D. A.","year":"1996","journal-title":"Cell"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1146\/annurev.cellbio.011209.122036","article-title":"Mechanical integration of actin and adhesion dynamics in cell migration","volume":"26","author":"Gardel M. L.","year":"2010","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"1012","DOI":"10.1016\/j.cell.2011.06.010","article-title":"Life at the leading edge","volume":"145","author":"Ridley A. J.","year":"2011","journal-title":"Cell"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1038\/385537a0","article-title":"Integrin\u2010ligand binding properties govern cell migration speed through cell\u2010substratum adhesiveness","volume":"385","author":"Palecek S. P.","year":"1997","journal-title":"Nature"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1083\/jcb.200909003","article-title":"Plasticity of cell migration: A multiscale tuning model","volume":"188","author":"Friedl P.","year":"2009","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1083\/jcb.200810041","article-title":"One\u2010dimensional topography underlies three\u2010dimensional fibrillar cell migration","volume":"184","author":"Doyle A. D.","year":"2009","journal-title":"Journal of Cell Biology"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"5917","DOI":"10.1242\/jcs.093732","article-title":"At the leading edge of three\u2010dimensional cell migration","volume":"125","author":"Petrie R. J.","year":"2012","journal-title":"Journal of Cell Science"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1038\/s41580-019-0172-9","article-title":"Mechanisms of 3D cell migration","volume":"20","author":"Yamada K. M.","year":"2019","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.bpj.2012.12.001","article-title":"Guidance of cell migration by substrate dimension","volume":"104","author":"Chang S. S.","year":"2013","journal-title":"Biophysical Journal"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"3580","DOI":"10.1039\/b911967a","article-title":"Rapid multicomponent optical protein patterning","volume":"9","author":"B\u00e9lisle J. M.","year":"2009","journal-title":"Lab on a Chip"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","first-page":"5257","DOI":"10.1002\/adma.201301762","article-title":"Cell\u2013material interactions revealed via material techniques of surface patterning","volume":"25","author":"Yao X.","year":"2013","journal-title":"Advanced Materials"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"2024","DOI":"10.1002\/adma.201504154","article-title":"Multiprotein printing by light\u2010induced molecular adsorption","volume":"28","author":"Strale P.\u2010O.","year":"2016","journal-title":"Advanced Materials"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"4201","DOI":"10.1242\/jcs.075150","article-title":"Micropatterning as a tool to decipher cell morphogenesis and functions","volume":"123","author":"Th\u00e9ry M.","year":"2010","journal-title":"Journal of Cell Science"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2024.1352279","article-title":"Mesenchymal cell migration on one\u2010dimensional micropatterns","volume":"12","author":"Heyn J. C. J.","year":"2024","journal-title":"Frontiers in Cell and Developmental Biology"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","unstructured":"Mi X. Zhang Y. &Klausen L. H.(2025).Elucidating micropatterned cellular mechanics via integrating optical microscopy and atomic force microscopy. In:2025 IEEE International Conference on Manipulation Manufacturing and Measurement on the Nanoscale (3M\u2010NANO). IEEE.","DOI":"10.1109\/3M-NANO65639.2025.11261169"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1093\/pnasnexus\/pgad237","article-title":"Physical forces guide curvature sensing and cell migration mode bifurcating","volume":"2","author":"Feng L.","year":"2023","journal-title":"PNAS Nexus"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"3995","DOI":"10.1038\/s41467-018-06494-6","article-title":"Curvotaxis directs cell migration through cell\u2010scale curvature landscapes","volume":"9","author":"Pieuchot L.","year":"2018","journal-title":"Nature Communications"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","DOI":"10.1002\/advs.201600347","article-title":"Surface curvature differentially regulates stem cell migration and differentiation via altered attachment morphology and nuclear deformation","volume":"4","author":"Werner M.","year":"2017","journal-title":"Advanced Science"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"4118","DOI":"10.1038\/s41467-021-24249-8","article-title":"Cell migration guided by long\u2010lived spatial memory","volume":"12","author":"d'Alessandro J.","year":"2021","journal-title":"Nature Communications"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","DOI":"10.1103\/PhysRevResearch.2.033237","article-title":"One\u2010dimensional cell motility patterns","volume":"2","author":"Ron J. E.","year":"2020","journal-title":"Physical Review Research"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2318248121","article-title":"Deposited footprints let cells switch between confined, oscillatory, and exploratory migration","volume":"121","author":"Perez Ipi\u00f1a E.","year":"2024","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1042\/EBC20190015","article-title":"Actin dynamics in cell migration","volume":"63","author":"Schaks M.","year":"2019","journal-title":"Essays in Biochemistry"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.cell.2011.10.050","article-title":"Membrane tension maintains cell polarity by confining signals to the leading edge during neutrophil migration","volume":"148","author":"Houk A. R.","year":"2012","journal-title":"Cell"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1016\/j.bpj.2019.07.022","article-title":"A biophysical model for curvature\u2010guided cell migration","volume":"117","author":"Vassaux M.","year":"2019","journal-title":"Biophysical Journal"},{"key":"e_1_2_8_33_1","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1038\/emboj.2010.2","article-title":"Force generated by actomyosin contraction builds bridges between adhesive contacts","volume":"29","author":"Rossier O. M.","year":"2010","journal-title":"The EMBO Journal"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","first-page":"2437","DOI":"10.1016\/j.bpj.2015.04.005","article-title":"Cell shape dynamics reveal balance of elasticity and contractility in peripheral arcs","volume":"108","author":"Labouesse C.","year":"2015","journal-title":"Biophysical Journal"}],"container-title":["Journal of Microscopy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jmi.70166","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jmi.70166","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jmi.70166","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T10:39:51Z","timestamp":1788518391000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jmi.70166"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":33,"alternative-id":["10.1111\/jmi.70166"],"URL":"https:\/\/doi.org\/10.1111\/jmi.70166","archive":["Portico"],"relation":{},"ISSN":["0022-2720","1365-2818"],"issn-type":[{"value":"0022-2720","type":"print"},{"value":"1365-2818","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]},"assertion":[{"value":"2026-06-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"jmi.70166"}}