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In addition, we assessed whether aromatase expression in <jats:styled-content style=\"fixed-case\">PBL<\/jats:styled-content>s could be used as a parameter of aromatase expression in other tissues, using the cynomolgus monkey as model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Real\u2010time PCR analysis of aromatase gene expression and protein evaluation by Western blot was performed in <jats:styled-content style=\"fixed-case\">PBL<\/jats:styled-content>s of human elderly subjects and in various tissues from cynomolgus monkeys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>No gender\u2010related difference in <jats:italic>CYP19A1<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein expression in PBLs from human elderly women and men was found. In elderly male cynomolgus monkeys, <jats:italic>CYP19A1<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and protein were expressed in all cells and tissues analysed, with the lowest levels in PBLs but no clear\u2010cut correlation with other tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Aromatase expression in <jats:styled-content style=\"fixed-case\">PBL<\/jats:styled-content>s in elderly human subjects is not gender\u2010related and cannot be a surrogate of aromatase expression for other tissues.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12000","type":"journal-article","created":{"date-parts":[[2012,9,17]],"date-time":"2012-09-17T06:12:02Z","timestamp":1347862322000},"page":"372-383","source":"Crossref","is-referenced-by-count":3,"title":["Aromatase expression in human peripheral blood leucocytes (<scp>PBL<\/scp>s) and in various tissues in primates: studies in elderly humans and cynomolgus 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