{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,19]],"date-time":"2024-09-19T15:32:12Z","timestamp":1726759932899},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T00:00:00Z","timestamp":1391558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Institute of Primate Research"},{"name":"Jomo Kenyatta University of Agriculture and Technology (JKUAT) Research Production and Extension Department"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Non\u2010human primates are important experimental models for human <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>frican trypanosomiasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Six monkeys were intravenously inoculated with 10<jats:sup>5<\/jats:sup> trypanosomes of <jats:italic><jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>rypanosoma brucei gambiense <\/jats:italic><jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content> 3253. They were monitored for 180\u00a0days for parasitemia, hematology, clinical and biochemical profiles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The pre\u2010patent period was 2\u20133\u00a0days. From 33 to 123\u00a0dpi, the parasitemia was low and only detectable by the hematocrit centrifugation technique. Thereafter, to the end of the experimental period, the parasitemia was undetectable by parasitological methods. Clinical signs observed were lymphadenopathy and splenomegaly. Hematological changes included a decline in hemoglobin occurring between 14 and 56\u00a0dpi and a significant decline in platelet counts after infection. The levels of total protein, albumin and globulins increased from 26\u00a0dpi for the rest of the experimental period. No parasites were detected in <jats:styled-content style=\"fixed-case\">cerebrospinal spinal fluid<\/jats:styled-content>, and no brain pathology was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This vervet monkey model can only be used for early\u2010stage disease <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ambian sleeping sickness.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12100","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T11:29:33Z","timestamp":1391599773000},"page":"72-77","source":"Crossref","is-referenced-by-count":3,"title":["<i><scp>T<\/scp>rypanosoma brucei gambiense<\/i> infection in vervet monkeys: a potential model for early\u2010stage disease"],"prefix":"10.1111","volume":"43","author":[{"given":"B.M.","family":"Gachie","sequence":"first","affiliation":[{"name":"Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Kagira","sequence":"additional","affiliation":[{"name":"Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.M.","family":"Karanja","sequence":"additional","affiliation":[{"name":"Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.W.","family":"Waema","sequence":"additional","affiliation":[{"name":"Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.M.","family":"Ngotho","sequence":"additional","affiliation":[{"name":"Institute of Primate Research Karen Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.W.N.","family":"Maina","sequence":"additional","affiliation":[{"name":"Jomo Kenyatta University of Agriculture and Technology Nairobi Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,5]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"187","article-title":"Serum total proteins and creatinine levels in experimental Gambian trypanosomiasis of vervet monkeys","volume":"4","author":"Abenga JN","year":"2005","journal-title":"Afr J Biotechnol"},{"key":"e_1_2_6_3_1","first-page":"738","article-title":"The biochemical changes induced by natural human African trypanosome infections","volume":"5","author":"Awobode HO","year":"2006","journal-title":"Afr J Biotechnol"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)68591-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-2-8178-0857-4_13"},{"key":"e_1_2_6_6_1","doi-asserted-by":"crossref","first-page":"53","DOI":"10.19182\/remvt.9216","article-title":"Haematology of experimental Trypanosoma brucei gambiense infection. 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