{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T18:27:06Z","timestamp":1784831226335,"version":"3.55.0"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,9,14]],"date-time":"2015-09-14T00:00:00Z","timestamp":1442188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000774","name":"Defense Threat Reduction Agency","doi-asserted-by":"publisher","award":["1881290"],"award-info":[{"award-number":["1881290"]}],"id":[{"id":"10.13039\/100000774","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Moraxella macacae<\/jats:italic> is a recently described bacterial pathogen that causes epistaxis or so\u2010called bloody nose syndrome in captive macaques. The aim of this study was to develop specific molecular diagnostic assays for <jats:italic>M. macacae<\/jats:italic> and to determine their performance characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We developed six real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assays on the Roche LightCycler. The accuracy, precision, selectivity, and limit of detection (<jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content>) were determined for each assay, in addition to further validation by testing nasal swabs from macaques presenting with epistaxis at the Tulane National Primate Research Center.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All assays exhibited 100% specificity and were highly sensitive with an <jats:styled-content style=\"fixed-case\">LOD<\/jats:styled-content> of 10 fg for chromosomal assays and 1 fg for the plasmid assay. Testing of nasal swabs from 10 symptomatic macaques confirmed the presence of <jats:italic>M. macacae<\/jats:italic> in these animals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We developed several accurate, sensitive, and species\u2010specific real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> assays for the detection of <jats:italic>M. macacae<\/jats:italic> in captive macaques.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12196","type":"journal-article","created":{"date-parts":[[2015,9,14]],"date-time":"2015-09-14T07:14:48Z","timestamp":1442214888000},"page":"364-372","source":"Crossref","is-referenced-by-count":5,"title":["Development of real\u2010time <scp>PCR<\/scp> assays for the detection of <i>Moraxella macacae<\/i> associated with bloody nose syndrome in rhesus (<i>Macaca mulatta<\/i>) and cynomolgus (<i>Macaca fascicularis<\/i>) macaques"],"prefix":"10.1111","volume":"44","author":[{"given":"Chris A.","family":"Whitehouse","sequence":"first","affiliation":[{"name":"Molecular and Translational Sciences Division United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kitty","family":"Chase","sequence":"additional","affiliation":[{"name":"Diagnostic Systems Division United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica E.","family":"Embers","sequence":"additional","affiliation":[{"name":"Division of Bacteriology and Parasitology Tulane National Primate Research Center Tulane University Health Sciences Covington LA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Kulesh","sequence":"additional","affiliation":[{"name":"Diagnostic Systems Division United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason T.","family":"Ladner","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo F.","family":"Palacios","sequence":"additional","affiliation":[{"name":"Center for Genome Sciences United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy D.","family":"Minogue","sequence":"additional","affiliation":[{"name":"Diagnostic Systems Division United States Army Medical Research Institute of Infectious Diseases Fort Detrick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,14]]},"reference":[{"issue":"1","key":"e_1_2_6_2_1","first-page":"S49","article-title":"Limit of blank, 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