{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T02:23:52Z","timestamp":1788143032709,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T00:00:00Z","timestamp":1599004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Leptospirosis is an important anthropozoonosis. The study investigated the presence of anti\u2010<jats:italic>Leptospira<\/jats:italic> antibodies and detection of <jats:italic>Leptospira<\/jats:italic> spp DNA in the urine as well as the biochemical profile in Neotropical wild primates living in a forest reserve from Southeast S\u00e3o Paulo State, Brazil.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Blood samples were obtained from 50 adult tufted capuchin monkeys (<jats:italic>Cebus apella nigritus)<\/jats:italic>. Urine samples were obtained only from male primates. The screening for antibodies against <jats:italic>Leptospira<\/jats:italic> spp was evaluated by microscopic agglutination test (MAT). <jats:italic>Leptospira<\/jats:italic> DNA in the urine was evaluated by polymerase chain reaction (PCR) considering the target gene LipL32. Biochemical profile was evaluated by using a spectrophotometer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The MAT results included 39 (78%) serum reactive animals with the proportions of 28\/39 males and 11\/39 females. The most frequent reactive serogroups were Icterohemorrhagiae, Canicola, and Autumnalis. All urine samples were negative for leptospiral DNA. There were no significant differences between sexes for aspartate aminotransferase (AST) and alkaline phosphatase values, but alanine aminotransferase (ALT), creatinine, glucose, and urea were significantly higher in males.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Tufted capuchin monkeys were sera reactive against leptospirosis. Prevalence was similar for the 2 sexes. Leptospiral DNA was not detected in the urine of sera reactive primates tested by the MAT method. ALT, creatinine, glucose, and urea values were higher in male animals.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12493","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T13:09:40Z","timestamp":1599052180000},"page":"3-8","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Serological survey and DNA screening of <i>Leptospira<\/i> spp. in free\u2010living adult tufted capuchin monkeys (<i>Cebus apella nigritus<\/i>) in a forest reserve Southeast S\u00e3o Paulo State, Brazil"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8845-8985","authenticated-orcid":false,"given":"Raul Jos\u00e9 Silva","family":"Girio","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine Mar\u00edlia University Mar\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7430-0290","authenticated-orcid":false,"given":"Tatiana Morosini","family":"de Andrade\u2010Cruvinel","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine University Center of Rio Preto (Unirp) S\u00e3o Jos\u00e9 do Rio Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7070-7920","authenticated-orcid":false,"given":"Silvio Arruda","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine and Zootechnics S\u00e3o Paulo University S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9441-4647","authenticated-orcid":false,"given":"Cl\u00e1udia Sampaio Fonseca","family":"Repetti","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Mar\u00edlia University Mar\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6739-3932","authenticated-orcid":false,"given":"Milena","family":"Friolani","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Mar\u00edlia University Mar\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8964-9687","authenticated-orcid":false,"given":"Patr\u00edcia Cincotto dos Santos","family":"Bueno","sequence":"additional","affiliation":[{"name":"School of Medicine Mar\u00edlia University Mar\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5126-2540","authenticated-orcid":false,"given":"Marc\u00edlio","family":"Felix","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Mar\u00edlia University Mar\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8172-7911","authenticated-orcid":false,"given":"Daniel De Bortoli","family":"Teixeira","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Mar\u00edlia University Mar\u00edlia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"168","volume-title":"Primatas do Brasil","author":"Auricchio P","year":"1995"},{"key":"e_1_2_6_3_1","first-page":"339","volume-title":"The Complete Capuchin: The Biology of the Genus Cebus","author":"Fragaszy DM","year":"2004"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1896\/1413-4705.13.1.14"},{"key":"e_1_2_6_5_1","first-page":"331","volume-title":"Ecology and Behavior of Neotropical Primates","author":"Freese CH","year":"1981"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1006\/anbe.1996.0416"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph14111421"},{"key":"e_1_2_6_8_1","volume-title":"Leptospirose: \u00d3bitos de 2000\u20132019","author":"Brasil. 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