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This study describes an outbreak of toxoplasmosis in howler monkeys (<jats:italic>Alouatta<\/jats:italic> sp.) and survival of capuchins (<jats:italic>Sapajus apella<\/jats:italic>), under the same environmental conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Howler monkeys were submitted to post\u2010mortem examination. Tissue samples were processed to histopathology and immunohistochemistry to detect lesions and tachyzoites of <jats:italic>Toxoplasma gondii<\/jats:italic>. Tissue samples were also frozen and submitted to PCR and genotyping of <jats:italic>T. gondii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Typical lesions were observed in several organs including the liver, lymph node, and brain, with intralesional cysts and tachyzoites of <jats:italic>T. gondii<\/jats:italic> demonstrated by immunohistochemistry. <jats:italic>T. gondii<\/jats:italic> genomic sequences were amplified by PCR, and genotyping characterized the same <jats:italic>T. gondii<\/jats:italic> clone in all howler monkeys.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results support the notion that some species of neotropical primates are highly susceptible to toxoplasmosis and the hypothesis that capuchins (<jats:italic>S. apella<\/jats:italic>) may be resistant.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12506","type":"journal-article","created":{"date-parts":[[2020,12,7]],"date-time":"2020-12-07T04:43:30Z","timestamp":1607316210000},"page":"99-107","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Genotyping of <i>Toxoplasma gondii<\/i> in a lethal toxoplasmosis outbreak affecting captive howler monkeys (<i>Alouatta<\/i> sp.)"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2053-6278","authenticated-orcid":false,"given":"Clarissa Helena","family":"Santana","sequence":"first","affiliation":[{"name":"Escola de Veterin\u00e1ria Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1ria Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9031-792X","authenticated-orcid":false,"given":"Ayisa Rodrigues","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1ria Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3083-1572","authenticated-orcid":false,"given":"Daniel O.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1ria Universidade Federal de Minas Gerais (UFMG) Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5613-8373","authenticated-orcid":false,"given":"Samantha Pinheiro","family":"Pimentel","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1ria Universidade Federal de Minas Gerais (UFMG) Belo Horizonte 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