{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:31:57Z","timestamp":1747186317240,"version":"3.40.5"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,3,12]],"date-time":"2022-03-12T00:00:00Z","timestamp":1647043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Toxoplasma gondii<\/jats:italic> and <jats:italic>Neospora caninum<\/jats:italic> infections in primates are potentially fatal and directly impact the conservation of these animals and public health.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>A total of 38 blood\/clot samples collected from free\u2010living and captive neotropical primates undergoing clinical care or found dead by environmental authorities in the Mato Grosso State, Brazil, were analyzed by PCR for DNA detection of <jats:italic>T<\/jats:italic>.\u00a0<jats:italic>gondii<\/jats:italic> and <jats:italic>N<\/jats:italic>.\u00a0<jats:italic>caninum<\/jats:italic>. Furthermore, eight animals were submitted to immunohistochemistry for the detection of <jats:italic>T<\/jats:italic>.\u00a0<jats:italic>gondii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>DNA of <jats:italic>T<\/jats:italic>.\u00a0<jats:italic>gondii<\/jats:italic> and <jats:italic>N<\/jats:italic>.\u00a0<jats:italic>caninum<\/jats:italic> was amplified in 11 (28.95%) 10 (26.32%) of samples analyzed, respectively. Coinfection was observed in three individuals. One animal returned a positive result in the immunohistochemistry for the detection of <jats:italic>T<\/jats:italic>.\u00a0<jats:italic>gondii<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings reflect a concern for the conservation of these animals, as the pathogen\u2013host interaction is unpredictable and infections by these protozoa can lead to animal mortality, which has a substantial impact on endangered species.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12575","type":"journal-article","created":{"date-parts":[[2022,3,12]],"date-time":"2022-03-12T11:45:43Z","timestamp":1647085543000},"page":"149-154","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular detection of <i>Toxoplasma gondii<\/i> and <i>Neospora caninum<\/i> in neotropical 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