{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:32:05Z","timestamp":1747186325698,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T00:00:00Z","timestamp":1679875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003715","name":"Korea Research Institute of Bioscience and Biotechnology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003715","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004085","name":"Ministry of Education, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004085","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Melatonin is a multifunctional hormone synthesized in the pineal gland and peripheral reproductive tissues that regulates many biological processes. There is increasing evidence for a role of melatonin in oocyte maturation and embryonic development in various mammals. However, no study has reported evidence for the existence of melatonergic system, such as melatonin synthesis enzymes, melatonin membrane receptors, or melatonin binding sites in non\u2010human primate cumulus\u2013oocyte complexes (COCs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Reverse transcription polymerase chain reaction (RT\u2010PCR) and immunocytochemistry were performed to detect transcripts and proteins of the rate\u2010limiting enzyme in melatonin synthesis (arylalkylamine N\u2010acetyltransferase, AANAT), melatonin membrane receptors (MT1 and MT2), and a melatonin binding site (NRH: quinone oxidoreductase 2, NQO2) in cynomolgus monkey COCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>RT\u2010PCR analyses revealed the presence of <jats:italic>AANAT<\/jats:italic>, <jats:italic>MT1<\/jats:italic>, <jats:italic>MT2<\/jats:italic>, and <jats:italic>NQO2<\/jats:italic> transcripts in granulosa cells, germinal vesicle (GV)\u2010 and metaphase II (MII)\u2010stage cumulus cells, and oocytes. Immunocytochemistry revealed the presence of AANAT, MT1, MT2, and NQO2 proteins in GV\u2010 and MII\u2010stage COCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results provide the first evidence for the existence of the rate\u2010limiting enzyme required for melatonin synthesis, melatonin membrane receptors, and a melatonin binding site in non\u2010human primate COCs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jmp.12641","type":"journal-article","created":{"date-parts":[[2023,3,28]],"date-time":"2023-03-28T04:39:48Z","timestamp":1679978388000},"page":"163-169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression of the melatonergic system during meiotic maturation of cynomolgus monkey cumulus\u2013oocyte complexes"],"prefix":"10.1111","volume":"52","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1265-3821","authenticated-orcid":false,"given":"Sanghoon","family":"Lee","sequence":"first","affiliation":[{"name":"Futuristic Animal Resource &amp; Research Center Korea Research Institute of Bioscience and Biotechnology  Cheongju South\u00a0Korea"},{"name":"Laboratory of Theriogenology, College of Veterinary Medicine Chungnam National University  Daejeon South\u00a0Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyo\u2010Gu","family":"Kang","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource &amp; Research Center Korea Research Institute of Bioscience and Biotechnology  Cheongju South\u00a0Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pil\u2010Soo","family":"Jeong","sequence":"additional","affiliation":[{"name":"Futuristic Animal Resource &amp; Research Center Korea Research Institute of Bioscience and Biotechnology  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