{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T04:35:51Z","timestamp":1765514151235,"version":"3.48.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,11,23]],"date-time":"2025-11-23T00:00:00Z","timestamp":1763856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["400551\/2014\u20104"],"award-info":[{"award-number":["400551\/2014\u20104"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Medical Primatology"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The Peruvian Amazon is an endemic region for integumentary leishmaniasis, characterized by multiple vectors species, diverse wildlife reservoirs, and human activities that influence disease transmission. Non\u2010human primates (\n                      <jats:styled-content style=\"fixed-case\">NHPs<\/jats:styled-content>\n                      ) have been implicated as potential reservoirs for\n                      <jats:italic>Leishmania<\/jats:italic>\n                      spp. and\n                      <jats:italic>Trypanosoma cruzi<\/jats:italic>\n                      in neighboring Brazil, but their role in\n                      <jats:italic>Cacajao<\/jats:italic>\n                      spp. remains unknown. This study aimed to detect\n                      <jats:italic>Leishmania<\/jats:italic>\n                      spp. and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>T. cruzi<\/jats:italic>\n                        DNA\n                      <\/jats:styled-content>\n                      in semi\u2010captive Peruvian bald uakaris (\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Cacajao calvus<\/jats:italic>\n                      <\/jats:styled-content>\n                      ) housed at the Pilpintuwasi Rescue Center, Iquitos, Peru.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Seven individuals were chemically restrained, and blood samples were collected.\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      was extracted and analyzed using\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      with primers targeting the heat\u2010shock protein 70 (\n                      <jats:styled-content style=\"fixed-case\">HSP70<\/jats:styled-content>\n                      ) gene for\n                      <jats:italic>Leishmania<\/jats:italic>\n                      spp. and kinetoplast\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      (\n                      <jats:styled-content style=\"fixed-case\">kDNA<\/jats:styled-content>\n                      ) for\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>T. cruzi<\/jats:italic>\n                      <\/jats:styled-content>\n                      . Mitochondrial\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      sequencing using a novel\n                      <jats:styled-content style=\"fixed-case\">CacajaoID<\/jats:styled-content>\n                      primer set confirmed species and subspecies identity. Analytical sensitivity assays were also performed to ensure assay the reliability and detection limit of both\n                      <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                      protocols.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All specimens tested negative for both pathogens. Sequencing of the\n                      <jats:styled-content style=\"fixed-case\">CacajaoID<\/jats:styled-content>\n                      fragment confirmed five individuals as\n                      <jats:italic>C. c. ucayalii<\/jats:italic>\n                      and two as\n                      <jats:italic>C. c. novaesi<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Although no parasite\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      was detected, these findings provide baseline molecular and epidemiological Data for bald uakaris and highlight the importance of continued surveillance in semi\u2010captive and wild populations. The results support one health approaches by linking wildlife monitoring with conservation and zoonotic risk assessments in the Amazon region.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jmp.70046","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T04:44:51Z","timestamp":1763959491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epidemiological Surveillance of\n                    <i>Leishmania<\/i>\n                    spp. and\n                    <i>Trypanosoma cruzi<\/i>\n                    in Peruvian Bald Uakaris (\n                    <i>Cacajao calvus ucayalii<\/i>\n                    ) in a Peruvian Rescue Center"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8041-565X","authenticated-orcid":false,"given":"Pedro Paulo","family":"de Oliveira Nogueira","sequence":"first","affiliation":[{"name":"Department of Genetics, Microbiology and Immunology, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larissa Pereira","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Genetics, Microbiology and Immunology, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Orlando Beselly","family":"Cueva","sequence":"additional","affiliation":[{"name":"Department of Genetics, Microbiology and Immunology, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00edgia Souza Lima Silveira","family":"da Mota","sequence":"additional","affiliation":[{"name":"Department of Genetics, Microbiology and Immunology, Institute of Biosciences S\u00e3o Paulo State University (UNESP)  Botucatu Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006029"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2014.08.004"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jmp.12441"},{"key":"e_1_2_10_5_1","first-page":"69","article-title":"On the Geographic Distribution of the Bald Uakaris (Cacajao calvus ssp.) in Brazilian Amazonia","volume":"35","author":"Silva F.","year":"2021","journal-title":"Primate Conservation"},{"key":"e_1_2_10_6_1","unstructured":"A.Calouro \u201cAvalia\u00e7\u00e3o do risco de extin\u00e7\u00e3o deCacajao calvus ucayalii(Thomas 1928) no Brasil \u201d(2015) accessed 24 September 2021 https:\/\/www.icmbio.gov.br\/portal_antigo\/component\/content\/article.html?id=7285:mamiferos\u2010cacajao\u2010calvus\u2010ucayalii\u2010Uakari."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.15381\/rpb.v24i3.13907"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.24070\/bjvp.1983\u20100246.v16i1p1\u201034"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10393\u2010017\u20101271\u20108"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/s1043\u20104526(10)59003\u2010x"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.v34i4.2204"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.apar.2016.06.001"},{"volume-title":"Tratado de animais selvagens: medicina veterin\u00e1ria","year":"2014","author":"Cubas Z. 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