{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:58:16Z","timestamp":1761541096084,"version":"3.37.3"},"reference-count":43,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,9,28]],"date-time":"2012-09-28T00:00:00Z","timestamp":1348790400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009550","name":"Hasselt University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009550","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Charcot Foundation Belgium"},{"name":"Methusalem excellence grant of the Flemish Government"},{"DOI":"10.13039\/100006314","name":"Biogen-Idec","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006314","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Bayer"},{"name":"Merck-Serono"},{"DOI":"10.13039\/100006259","name":"TEVA","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006259","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004339","name":"Sanofi Aventis","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004339","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100004336","name":"NOVARTIS","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100004336","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2012,11]]},"abstract":"Abstract<\/jats:title>Limited information is available on the identity of antigens targeted by antibodies present in cerebrospinal fluid (CSF<\/jats:styled-content>) of patients with clinically isolated syndrome (CIS<\/jats:styled-content>). The aim of this study was to identify novel antigens for CIS<\/jats:styled-content> and investigate their prognostic potential to predict conversion to multiple sclerosis (MS<\/jats:styled-content>). We applied serological antigen selection (SAS<\/jats:styled-content>) to identify antigens interacting with antibodies present in the pooled CSF<\/jats:styled-content> from four CIS<\/jats:styled-content> patients, who developed MS<\/jats:styled-content>. Antibody reactivity towards CIS<\/jats:styled-content> antigens identified by SAS<\/jats:styled-content> was tested in CSF<\/jats:styled-content> and serum from patients with CIS<\/jats:styled-content> (n\u00a0<\/jats:italic>=\u00a0123\/n\u00a0<\/jats:italic>=\u00a0108), MS<\/jats:styled-content> (n\u00a0<\/jats:italic>=\u00a065\/n\u00a0<\/jats:italic>=\u00a044), and other (inflammatory) neurological diseases (n\u00a0<\/jats:italic>=\u00a075\/n\u00a0<\/jats:italic>=\u00a038) as well as in healthy control sera (n\u00a0<\/jats:italic>=\u00a044). Using SAS<\/jats:styled-content>, a panel of six novel CIS<\/jats:styled-content> candidate antigens was identified. CSF<\/jats:styled-content> antibody reactivity was detected in both CIS<\/jats:styled-content> and relapsing\u2010remitting (RR<\/jats:styled-content>) MS<\/jats:styled-content>. Serum reactivity was significantly increased in CIS<\/jats:styled-content> and RR<\/jats:styled-content>\u2010MS<\/jats:styled-content> as compared with controls (p\u00a0<\/jats:italic>=\u00a00.03). For two antigens, the frequency of antibody\u2010positive patients was higher in CIS<\/jats:styled-content> patients who converted to MS<\/jats:styled-content> as compared with CIS<\/jats:styled-content> patients without conversion. We identified novel CIS<\/jats:styled-content> antigens to which antibody reactivity was primarily detected in CIS<\/jats:styled-content> and RR<\/jats:styled-content>\u2010MS<\/jats:styled-content> as compared to controls. Possible prognostic potential could be demonstrated for two antigens.<\/jats:p>","DOI":"10.1111\/jnc.12005","type":"journal-article","created":{"date-parts":[[2012,9,7]],"date-time":"2012-09-07T12:32:46Z","timestamp":1347021166000},"page":"568-577","source":"Crossref","is-referenced-by-count":10,"title":["Novel cerebrospinal fluid and serum autoantibody targets for clinically isolated syndrome"],"prefix":"10.1111","volume":"123","author":[{"given":"Myrthe","family":"Rouwette","sequence":"first","affiliation":[{"name":"Hasselt University Biomedical Research Institute (BIOMED) and transnationale Universiteit Limburg School of Life Sciences Diepenbeek Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaartje","family":"Somers","sequence":"additional","affiliation":[{"name":"Hasselt University Biomedical Research Institute (BIOMED) and transnationale Universiteit Limburg School of Life Sciences Diepenbeek Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy","family":"Govarts","sequence":"additional","affiliation":[{"name":"Hasselt University Biomedical Research Institute (BIOMED) and transnationale Universiteit Limburg School of Life Sciences Diepenbeek Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter P.","family":"De Deyn","sequence":"additional","affiliation":[{"name":"Department of Neurology Middelheim Hospital Antwerp Belgium"},{"name":"Laboratory of Neurochemistry and Behaviour Department of Biomedical Sciences Institute Born Bunge University of Antwerp Antwerp Belgium"},{"name":"Department of Neurology University Medical Center Groningen Groningen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raymond","family":"Hupperts","sequence":"additional","affiliation":[{"name":"School of Mental Health and Neuroscience Maastricht University Medical Center Maastricht the Netherlands"},{"name":"Department of Neurology Orbis Medical Center Sittard The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bart","family":"Van Wijmeersch","sequence":"additional","affiliation":[{"name":"Hasselt University Biomedical Research Institute (BIOMED) and transnationale Universiteit Limburg School of Life Sciences Diepenbeek Belgium"},{"name":"Multiple Sclerosis and Rehabilitation Center Overpelt Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigit A.","family":"De Jong","sequence":"additional","affiliation":[{"name":"MS Centre Nijmegen (MSCN) and Department of Neurology Radboud University Medical Centre Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel M.","family":"Verbeek","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Laboratory Medicine Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Van Pesch","sequence":"additional","affiliation":[{"name":"Department of Neurology Cliniques Universitaires St\u2010Luc Universit\u00e9 Catholique de Louvain Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Sindic","sequence":"additional","affiliation":[{"name":"Department of Neurology Cliniques Universitaires St\u2010Luc Universit\u00e9 Catholique de Louvain Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luisa M.","family":"Villar","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Immunology Ram\u00f3n y Cajal Hospital Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 C.","family":"\u00c1lvarez\u2010Cerme\u00f1o","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Immunology Ram\u00f3n y Cajal Hospital Madrid 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