{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:00:27Z","timestamp":1793293227167,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T00:00:00Z","timestamp":1372118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["OD011092"],"award-info":[{"award-number":["OD011092"]}]},{"name":"NIH","award":["HL087710"],"award-info":[{"award-number":["HL087710"]}]},{"name":"NIH","award":["CA151601"],"award-info":[{"award-number":["CA151601"]}]},{"name":"American Thoracic Society\/Boehringer Ingelheim","award":["ACR07-002"],"award-info":[{"award-number":["ACR07-002"]}],"id":[{"id":"https:\/\/ror.org\/0547sz392","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Abstract<\/jats:title>\n \n Synthesis of acetylcholine (\n AC<\/jats:styled-content>\n h) by non\u2010neuronal cells is now well established and plays diverse physiologic roles. In neurons, the Na\n +<\/jats:sup>\n \u2010dependent, high affinity choline transporter (\n CHT<\/jats:styled-content>\n 1) is absolutely required for\n AC<\/jats:styled-content>\n h synthesis. In contrast, some non\u2010neuronal cells synthesize\n AC<\/jats:styled-content>\n h in the absence of\n CHT<\/jats:styled-content>\n 1 indicating a fundamental difference in\n AC<\/jats:styled-content>\n h synthesis compared to neurons. The aim of this study was to identify choline transporters, other than\n CHT<\/jats:styled-content>\n 1, that play a role in non\u2010neuronal\n AC<\/jats:styled-content>\n h synthesis.\n AC<\/jats:styled-content>\n h synthesis was studied in lung and colon cancer cell lines focusing on the choline transporter\u2010like proteins, a five gene family choline\u2010transporter like protein (\n CTL<\/jats:styled-content>\n )1\u20135. Supporting a role for\n CTL<\/jats:styled-content>\n s in choline transport in lung cancer cells, choline transport was Na\n +<\/jats:sup>\n \u2010independent and\n CTL<\/jats:styled-content>\n 1\u20135 were expressed in all cells examined.\n CTL<\/jats:styled-content>\n 1, 2, and 5 were expressed at highest levels and knockdown of\n CTL<\/jats:styled-content>\n 1, 2, and 5 decreased choline transport in H82 lung cancer cells. Knockdowns of\n CTL<\/jats:styled-content>\n 1, 2, 3, and 5 had no effect on\n AC<\/jats:styled-content>\n h synthesis in H82 cells. In contrast, knockdown of\n CTL<\/jats:styled-content>\n 4 significantly decreased\n AC<\/jats:styled-content>\n h secretion by both lung and colon cancer cells. Conversely, increasing expression of\n CTL<\/jats:styled-content>\n 4 increased\n AC<\/jats:styled-content>\n h secretion. These results indicate that\n CTL<\/jats:styled-content>\n 4 mediates\n AC<\/jats:styled-content>\n h synthesis in non\u2010neuronal cell lines and presents a mechanism to target non\u2010neuronal\n AC<\/jats:styled-content>\n h synthesis without affecting neuronal\n AC<\/jats:styled-content>\n h synthesis.\n <\/jats:p>","DOI":"10.1111\/jnc.12298","type":"journal-article","created":{"date-parts":[[2013,5,8]],"date-time":"2013-05-08T07:38:56Z","timestamp":1367998736000},"page":"451-461","source":"Crossref","is-referenced-by-count":40,"title":["Choline transporter\u2010like protein 4 (\n CTL<\/scp>\n 4) links to non\u2010neuronal acetylcholine synthesis"],"prefix":"10.1111","volume":"126","author":[{"given":"Pingfang","family":"Song","sequence":"first","affiliation":[{"name":"Division of Neuroscience Oregon National Primate Research Center Oregon Health & Science University Beaverton Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen 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