{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:14:33Z","timestamp":1790500473755,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,11,13]],"date-time":"2013-11-13T00:00:00Z","timestamp":1384300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["R01NS060879"],"award-info":[{"award-number":["R01NS060879"]}]},{"name":"NSF","award":["IOS-0824393"],"award-info":[{"award-number":["IOS-0824393"]}]},{"DOI":"10.13039\/100009670","name":"NARSAD","doi-asserted-by":"crossref","award":["2006YI"],"award-info":[{"award-number":["2006YI"]}],"id":[{"id":"10.13039\/100009670","id-type":"DOI","asserted-by":"crossref"}]},{"name":"LSU REF"},{"name":"NIDCD Intramural Research Program"},{"DOI":"10.13039\/100001962","name":"Ataxia-Telangiectasia Children's Project","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001962","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Protein phosphorylation plays a critical role in neuronal transcription, translation, cell viability, and synaptic plasticity. In neurons, phospho\u2010enzymes and specific substrates directly link glutamate release and post\u2010synaptic depolarization to these cellular functions; however, many of these enzymes and their protein substrates remain uncharacterized or unidentified. In this article, we identify a novel, synaptically driven neuronal phosphoproteome characterized by a specific motif of serine\/threonine\u2010glutamine ([S\/T]\u2010Q, abbreviated as SQ). These SQ\u2010containing substrates are predominantly localized to dendrites, synapses, the soma; and activation of this SQ phosphoproteome by bicuculline application is induced via calcium influx through L\u2010type calcium channels. On the other hand, acute application of NMDA can inactivate this SQ phosphoproteome. We demonstrate that the SQ motif kinase Ataxia\u2010telangiectasia mutated can also localize to dendrites and dendritic spines, in addition to other subcellular compartments, and is activated by bicuculline application. Pharmacology studies indicate that Ataxia\u2010telangiectasia mutated and its sister kinase ataxia telangiectasia mutated and Rad3\u2010related up\u2010regulate these neuronal SQ substrates. Phosphoproteomics identified over 150 SQ\u2010containing substrates whose phosphorylation is bidirectionally regulated by synaptic activity.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc12487-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><jats:p>We discovered a novel phosphoproteome in neurons which is characterized by the phosphorylation of a specific motif (serine\/threonine\u2010glutamine, abbreviated as SQ). The SQ phosphoproteome is activated directly by synaptic activity\u2010mediated Ca<jats:sup>2+<\/jats:sup> influx from L\u2010type calcium channels, and is localized to multiple subcellular domains, including dendritic shafts\/spines. Substrates identified in this phosphoproteome exhibit diverse subcellular localizations and functions.<\/jats:p>","DOI":"10.1111\/jnc.12487","type":"journal-article","created":{"date-parts":[[2013,10,14]],"date-time":"2013-10-14T02:20:39Z","timestamp":1381717239000},"page":"841-851","source":"Crossref","is-referenced-by-count":25,"title":["Synaptic activity bidirectionally regulates a novel sequence\u2010specific S\u2010Q phosphoproteome in neurons"],"prefix":"10.1111","volume":"128","author":[{"given":"Benjamin","family":"Siddoway","sequence":"first","affiliation":[{"name":"Neuroscience Center Louisiana State University Health Sciences Center New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailong","family":"Hou","sequence":"additional","affiliation":[{"name":"Neuroscience Center Louisiana State University Health Sciences Center New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongtian","family":"Yang","sequence":"additional","affiliation":[{"name":"Neuroscience Center Louisiana State University Health Sciences Center New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Petralia","sequence":"additional","affiliation":[{"name":"National Institute on Deafness and Other Communication Disorders NIH Bethesda Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houhui","family":"Xia","sequence":"additional","affiliation":[{"name":"Neuroscience Center Louisiana State University Health Sciences Center New Orleans Louisiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,13]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2004.04.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.4066-09.2010"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80086-0"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/lm.027201.112"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04254.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.7.3336"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201204035"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(03)00076-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2011.10.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1013"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2715"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.367"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2009.11.003"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2011.10.011"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1385\/MN:34:3:205"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000412"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00004.2008"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.10.039"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.17.10146"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn753"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2474"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5435.1870"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1140321"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.01.022"},{"key":"e_1_2_7_26_1","first-page":"4375","article-title":"Inhibition of phosphoinositide 3\u2010kinase related kinases by the radiosensitizing agent wortmannin","volume":"58","author":"Sarkaria J. 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