{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:33:09Z","timestamp":1791289989069,"version":"4.3.0"},"reference-count":30,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Health, Labour and Welfare of Japan"},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, and Science and Technology of Japan","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Japan Foundation for Pediatric Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">SHANK<\/jats:styled-content>3 is a synaptic scaffolding protein enriched in the post\u2010synaptic density of excitatory synapses. Since several <jats:styled-content style=\"fixed-case\">SHANK<\/jats:styled-content><jats:italic>3<\/jats:italic> mutations have been identified in a particular phenotypic group of patients with autism spectrum disorder (<jats:styled-content style=\"fixed-case\">ASD<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">SHANK<\/jats:styled-content>3 is strongly suspected of being involved in the pathogenesis and neuropathology of <jats:styled-content style=\"fixed-case\">ASD<\/jats:styled-content>. Several <jats:styled-content style=\"fixed-case\">SHANK<\/jats:styled-content>3 isoforms are known to be produced in the developing brain, but they have not been fully investigated. Here, we identified two different amino\u2010terminus truncated <jats:italic>Shank3<\/jats:italic> transcripts. One transcript, designated as <jats:italic>Shank3c\u20103<\/jats:italic>, produces an isoform that contains the entire carboxyl\u2010terminus, but the other transcript, designated as <jats:italic>Shank3c\u20104<\/jats:italic>, produces a carboxyl\u2010terminus truncated isoform. During development, expression of the novel <jats:italic>Shank3<\/jats:italic> transcripts increased after birth, transiently decreased at P14 and then gradually increased again thereafter. We also determined that methyl CpG\u2010binding protein 2 (Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2) is involved in regulating expression of the novel <jats:italic>Shank3<\/jats:italic> transcripts. Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2 is a transcriptional regulator that has been identified as the causative molecule of Rett syndrome, a neurodevelopmental disorder that includes autistic behavior. We demonstrated a difference between the expression of the novel <jats:italic>Shank3<\/jats:italic> transcripts in wild\u2010type mice and <jats:italic>Mecp2<\/jats:italic>\u2010deficient mice. These findings suggest that the <jats:styled-content style=\"fixed-case\">SHANK<\/jats:styled-content>3 isoforms may be implicated in the synaptic abnormality in Rett syndrome.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc12505-fig-0010-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><jats:p>SHANK3 is a synaptic scaffolding protein and is suspected of being implicated in the pathogenesis and neuropathology of ASD. We here identified two different amino\u2010terminus truncated <jats:italic>Shank3<\/jats:italic> transcripts, <jats:italic>Shank3c\u20103<\/jats:italic> and <jats:italic>Shank3c\u20104<\/jats:italic>, expressed from the intron 10 of the <jats:italic>Shank3<\/jats:italic> gene, and also suggested the epigenetic regulation of their expression via methyl CpG\u2010binding protein 2 (MeCP2) that has been identified as the causative molecule of Rett syndrome.<\/jats:p>","DOI":"10.1111\/jnc.12505","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T16:52:05Z","timestamp":1382633525000},"page":"280-293","source":"Crossref","is-referenced-by-count":22,"title":["Identification of two novel <i>Shank3<\/i> transcripts in the developing mouse neocortex"],"prefix":"10.1111","volume":"128","author":[{"given":"Chikako","family":"Waga","sequence":"first","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"},{"name":"Department of Mental Retardation and Birth Defect Research National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirotsugu","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomomi","family":"Sanagi","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Tsuchiya","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Itoh","sequence":"additional","affiliation":[{"name":"Department of Mental Retardation and Birth Defect Research National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010ichi","family":"Goto","sequence":"additional","affiliation":[{"name":"Department of Mental Retardation and Birth Defect Research National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Kohsaka","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeo","family":"Uchino","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry National Institute of Neuroscience Kodaira Tokyo Japan"},{"name":"Department of Biosciences School of Science and Engineering Teikyo University Utsunomiya Tochigi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/13810"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04539.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcn.2004.01.009"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2004.02910.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1086\/321293"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/2040-2392-1-15"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1153252"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1563"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1933"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2008.05.073"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnen\/64.6.537"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.b.30822"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/85899"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10795"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1139\/O08-115"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.03.016"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.37.12.e41"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.41.29510"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09165"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1086\/522590"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0896-6273(00)80809-0"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09965"},{"key":"e_1_2_7_25_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/nature11015","article-title":"Autistic\u2010like behaviours and hyperactivity in mice lacking ProSAP1\/Shank2","volume":"486","author":"Schmeisser M. J.","year":"2012","journal-title":"Nature"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.11.1851"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.03831.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/YPG.0b013e328341e069"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddr212"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1351604"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.6107-11.2012"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.12505","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.12505","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.12505","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T21:42:25Z","timestamp":1696282945000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.12505"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,14]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/jnc.12505"],"URL":"https:\/\/doi.org\/10.1111\/jnc.12505","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,14]]}}}