{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:28:21Z","timestamp":1791293301815,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T00:00:00Z","timestamp":1385337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"973 Program of China","award":["2011CBA00400"],"award-info":[{"award-number":["2011CBA00400"]}]},{"name":"CAS Hundreds of Talents Program"},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"crossref","award":["XDB02050400"],"award-info":[{"award-number":["XDB02050400"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002367","name":"Chinese Academy of Sciences","doi-asserted-by":"crossref","award":["SKLN-2010A08"],"award-info":[{"award-number":["SKLN-2010A08"]}],"id":[{"id":"10.13039\/501100002367","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Methyl CpG binding protein 2 (MeCP2) binds to methylated <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> and acts as a transcriptional repressor. Mutations of human <jats:italic><jats:styled-content style=\"fixed-case\">MECP<\/jats:styled-content>2<\/jats:italic> gene lead to Rett syndrome, a severe neural developmental disorder. Here, we report that the MeCP2 protein can be modified by covalent linkage to small ubiquitin\u2010like modifier (<jats:styled-content style=\"fixed-case\">SUMO<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">SUMO<\/jats:styled-content>ylation at lysine 223 is necessary for its transcriptional repression function. <jats:styled-content style=\"fixed-case\">SUMO<\/jats:styled-content>ylation of MeCP2 is required for the recruitment of histone deacetylase complexes 1\/2 complex. Mutation of Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2 lysine 223 to arginine abolishes its suppression of gene expression in mouse primary cortical neurons. Significantly, mutation of Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2\u00a0K223 site leads to developmental deficiency of rat hippocampal synapses <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. Thus, the <jats:styled-content style=\"fixed-case\">SUMO<\/jats:styled-content>ylation of Me<jats:styled-content style=\"fixed-case\">CP<\/jats:styled-content>2 at K223 is a critical switch for transcriptional repression and plays a crucial function in regulating synaptic development in the central nervous system.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc12523-fig-0005-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><jats:p>The Methyl CpG binding protein 2 (MeCP2) is implicated in Rett syndrome and autism spectrum disorders. We showed that MeCP2 protein could be modified by SUMOylation and SUMOylation of MeCP2 is critical for its transcriptional regulation activity. Furthermore, we found that SUMOylation of MeCP2 plays a critical role in regulating synapse development <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jnc.12523","type":"journal-article","created":{"date-parts":[[2013,11,5]],"date-time":"2013-11-05T12:45:59Z","timestamp":1383655559000},"page":"798-806","source":"Crossref","is-referenced-by-count":55,"title":["<scp>SUMO<\/scp>ylation of Me<scp>CP<\/scp>2 is essential for transcriptional repression and hippocampal synapse development"],"prefix":"10.1111","volume":"128","author":[{"given":"Ju","family":"Cheng","sequence":"first","affiliation":[{"name":"Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010Juan","family":"Xin","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun\u2010Ke","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Cell Biology &amp; Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine (SJTU\u2010SM) Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Xiu","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Cell Biology &amp; Shanghai Key Laboratory of Tumor Microenvironment and Inflammation Shanghai Jiao Tong University School of Medicine (SJTU\u2010SM) Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Hao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neonatology Children's Hospital of Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zilong","family":"Qiu","sequence":"additional","affiliation":[{"name":"Institute of Neuroscience Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/13810"},{"key":"e_1_2_7_3_1"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1153252"},{"key":"e_1_2_7_5_1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09582"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1086446"},{"key":"e_1_2_7_8_1"},{"key":"e_1_2_7_9_1"},{"key":"e_1_2_7_10_1"},{"key":"e_1_2_7_11_1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2866"},{"key":"e_1_2_7_13_1"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/30764"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0867\u201007.2007"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1122513"},{"key":"e_1_2_7_17_1"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811648106"},{"key":"e_1_2_7_19_1"},{"key":"e_1_2_7_20_1"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.4161\/epi.20733"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.12523","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.12523","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.12523","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T21:40:34Z","timestamp":1696196434000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.12523"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,25]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/jnc.12523"],"URL":"https:\/\/doi.org\/10.1111\/jnc.12523","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"value":"0022-3042","type":"print"},{"value":"1471-4159","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,25]]}}}