{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T01:31:23Z","timestamp":1790040683261,"version":"4.0.1"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,2]],"date-time":"2013-12-02T00:00:00Z","timestamp":1385942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005863","name":"National Brain Research Centre","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005863","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Council of Scientific & Industrial Research"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Japanese encephalitis virus (JEV), a single\u2010stranded RNA (ssRNA) virus, is the leading cause of encephalitis in Asia. Microglial activation is one of the key events in JEV\u2010induced neuroinflammation. Although the various microRNAs (miRNAs) has been shown to regulate microglia activation during pathological conditions including neuroviral infections, till date, the involvement of miRNAs in JEV infection has not been evaluated. Hence, we sought to evaluate the possible role of miRNAs in mediating JEV\u2010induced microglia activation. Initial screening revealed significant up\u2010regulation of miR\u201029b in JEV\u2010infected mouse microglial cell line (BV\u20102) and primary microglial cells. Furthermore, using bioinformatics tools, we identified tumor necrosis factor alpha\u2010induced protein 3, a negative regulator of nuclear factor\u2010kappa B signaling as a potential target of miR\u201029b. Interestingly, <jats:italic>in vitro<\/jats:italic> knockdown of miR\u201029b resulted in significant over\u2010expression of tumor necrosis factor alpha\u2010induced protein 3, and subsequent decrease in nuclear translocation of pNF\u2010\u03baB. JEV infection in BV\u20102 cell line elevated inducible nitric oxide synthase, cyclooxygenase\u20102, and pro\u2010inflammatory cytokine expression levels, which diminished after miR\u201029b knockdown. Collectively, our study demonstrates involvement of miR\u201029b in regulating JEV\u2010 induced microglial activation.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc12609-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><jats:p>miR\u201029b regulates Japanese Encephalitis Virus (JEV)\u2010induced microglia activation via inhibition of the anti\u2010inflammatory proteinTNFAIP3, which results in sustained activation of NF\u2010kB. Sustained NF\u2010?Bactivation further results in augmented secretion of pro\u2010inflammatory cytokines and induction of inflammatory mediators (iNOS and Cox\u20102).<\/jats:p>","DOI":"10.1111\/jnc.12609","type":"journal-article","created":{"date-parts":[[2013,11,16]],"date-time":"2013-11-16T10:36:41Z","timestamp":1384598201000},"page":"143-154","source":"Crossref","is-referenced-by-count":96,"title":["Micro<scp>RNA<\/scp>\u201029b modulates Japanese encephalitis virus\u2010induced microglia activation by targeting tumor necrosis factor alpha\u2010induced protein 3"],"prefix":"10.1111","volume":"129","author":[{"given":"Menaka Chanu","family":"Thounaojam","sequence":"first","affiliation":[{"name":"National Brain Research Centre Manesar Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak Kumar","family":"Kaushik","sequence":"additional","affiliation":[{"name":"National Brain Research Centre Manesar Haryana 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