{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:59:44Z","timestamp":1788872384481,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,24]],"date-time":"2014-02-24T00:00:00Z","timestamp":1393200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006327","name":"National Institute of Radiological Sciences","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006327","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    We developed the novel positron emission tomography (PET) ligand 2\u2010[5\u2010(4\u2010[\n                    <jats:sup>11<\/jats:sup>\n                    C]methoxyphenyl)\u20102\u2010oxo\u20101,3\u2010benzoxazol\u20103(\n                    <jats:italic>2H<\/jats:italic>\n                    )\u2010yl]\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyl\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010phenylacetamide ([\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP) for translocator protein (18\u00a0kDa, TSPO) imaging and evaluated its efficacy in ischemic rat brains. [\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP was synthesized by reacting desmethyl precursor (\n                    <jats:bold>1<\/jats:bold>\n                    ) with [\n                    <jats:sup>11<\/jats:sup>\n                    C]CH\n                    <jats:sub>3<\/jats:sub>\n                    I in radiochemical purity of \u2265\u00a098% and specific activity of 85\u00a0\u00b1\u00a030\u00a0GBq\/\u03bcmol (\n                    <jats:italic>n\u00a0=<\/jats:italic>\n                    \u00a018) at the end of synthesis. Biodistribution study on mice showed high accumulation of radioactivity in the TSPO\u2010rich organs, e.g., the lungs, heart, kidneys, and adrenal glands. The metabolite analysis in mice brain homogenate showed 80.1\u00a0\u00b1\u00a02.7% intact [\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP at 60\u00a0min after injection. To determine the specific binding of [\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP with TSPO in the brain,\n                    <jats:italic>in vitro<\/jats:italic>\n                    autoradiography and PET studies were performed in an ischemic rat model.\n                    <jats:italic>In vitro<\/jats:italic>\n                    autoradiography indicated significantly increased binding on the ipsilateral side compared with that on the contralateral side of ischemic rat brains. This result was supported firmly by the contrast of radioactivity between the ipsilateral and contralateral sides in PET images. Displacement experiments with unlabelled MBMP or PK11195 minimized the difference in uptake between the two sides. In summary, [\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP is a potential PET imaging agent for TSPO and, consequently, for the up\u2010regulation of microglia during neuroinflammation.\n                    <jats:boxed-text content-type=\"graphic\" position=\"anchor\">\n                      <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc12670-fig-0005-m.png\">\n                        <jats:alt-text>image<\/jats:alt-text>\n                      <\/jats:graphic>\n                    <\/jats:boxed-text>\n                  <\/jats:p>\n                  <jats:p>\n                    We developed 2\u2010[5\u2010(4\u2010[\n                    <jats:sup>11<\/jats:sup>\n                    C]methoxyphenyl)\u20102\u2010oxo\u20101,3\u2010benzoxazol\u20103(\n                    <jats:italic>2H<\/jats:italic>\n                    )\u2010yl]\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010methyl\u2010\n                    <jats:italic>N<\/jats:italic>\n                    \u2010phenylacetamide ([\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP) as a novel positron emission tomography ligand for imaging of translocator protein (18 kDa, TSPO) in the brain. [\n                    <jats:sup>11<\/jats:sup>\n                    C]MBMP exhibited high\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    specific binding with TSPO in the ischemic rat brain.\n                  <\/jats:p>","DOI":"10.1111\/jnc.12670","type":"journal-article","created":{"date-parts":[[2014,1,31]],"date-time":"2014-01-31T23:57:09Z","timestamp":1391212629000},"page":"712-720","source":"Crossref","is-referenced-by-count":45,"title":["Characterization of a novel acetamidobenzoxazolone\u2010based PET ligand for translocator protein (18\u00a0kDa) imaging of neuroinflammation in the brain"],"prefix":"10.1111","volume":"129","author":[{"given":"Anjani K.","family":"Tiwari","sequence":"first","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"},{"name":"Division of Cyclotron and Radiopharmaceutical Sciences Institute of Nuclear Medicine and Allied Sciences Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joji","family":"Yui","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Fujinaga","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katsushi","family":"Kumata","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoko","family":"Shimoda","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoteru","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Xie","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiko","family":"Hatori","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Maeda","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuki","family":"Nengaki","sequence":"additional","affiliation":[{"name":"Molecular Imaging Center National Institute of Radiological Sciences Chiba Japan"},{"name":"SHI Accelerator Service Co. 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