{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T14:27:17Z","timestamp":1774103237859,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,5,4]],"date-time":"2015-05-04T00:00:00Z","timestamp":1430697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81370473"],"award-info":[{"award-number":["81370473"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Natural Science Foundation of Jiangsu Province of China","award":["BK20131388"],"award-info":[{"award-number":["BK20131388"]}]},{"name":"Scientific Research Project of Jiangsu Provincial Department of Health","award":["H201342"],"award-info":[{"award-number":["H201342"]}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent studies have emphasized the important role of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) clusters and common target genes in disease progression. Despite the known involvement of the miR\u2010192\/215 family in many human diseases, its biological role in Hirschsprung disease (<jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>) remains undefined. In this study, we explored the role of the miR\u2010192\/215 family in the pathogenesis of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>. Quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and western blotting measured relative expression levels of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s, and proteins in 80 <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> patients and\u00a077 normal colon tissues. Targets were evaluated by dual\u2010luciferase reporter assays, and the functional effects of miR\u2010192\/215 on human 293T and <jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SY<\/jats:styled-content>5Y cells were detected by the Transwell assay, <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8 assay and flow cytometry. MiR\u2010192\/215 was significantly down\u2010regulated in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> tissue samples, and their knockdown inhibited cell migration and proliferation in the human 293T and <jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SY<\/jats:styled-content>5Y cell lines. Nidogen 1 (<jats:styled-content style=\"fixed-case\">NID<\/jats:styled-content>1) was confirmed as a common target gene of miR\u2010192\/215 by dual\u2010luciferase reporter gene assay and its expression was inversely correlated with that of miR\u2010192\/215 in tissue samples and cell lines. Silencing of\u00a0<jats:styled-content style=\"fixed-case\">NID<\/jats:styled-content>1 could rescue the extent of the suppressing effects by miR\u2010192\/215 inhibitor. The down\u2010regulation of miR\u2010192\/215 may contribute to <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> development by targeting <jats:styled-content style=\"fixed-case\">NID<\/jats:styled-content>1.\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13118-fig-0004-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\nWe proposed the following cascade for the proposed mechanism of miR\u2010192\/215 in the pathogenesis of Hirschsprung disease (HSCR) by targeting Nidogen 1 (NID1). Aberrant expression of miR\u2010192\/215 inhibits cell migration and cell proliferation via NID1. We think the miR\u2010192\/miR\u2010215\/NID1 signaling pathway may play an important role in the pathogenesis of HSCR.<\/jats:p>","DOI":"10.1111\/jnc.13118","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T07:03:12Z","timestamp":1428649392000},"page":"39-46","source":"Crossref","is-referenced-by-count":31,"title":["Nidogen\u20101 is a common target of micro<scp>RNA<\/scp>s MiR\u2010192\/215 in the pathogenesis of Hirschsprung's disease"],"prefix":"10.1111","volume":"134","author":[{"given":"Dongmei","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongxing","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hairong","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chao","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pingfa","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Surgery Nanjing Children Hospital Affiliated Nanjing Medical University Nanjing China"},{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ankur","family":"Sharan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of Public Health Nanjing Medical University Nanjing China"},{"name":"Key Laboratory of Modern Toxicology (Nanjing Medical University) Ministry of Education China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yankai","family":"Xia","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine Institute of Toxicology, School of 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