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This study measured peptide levels in the pituitary gland (Pit) and lateral hypothalamus (<jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content>) in adolescent (age 30\u00a0days) and adult (age 65\u00a0days) mice from four standard inbred strains,<jats:styled-content style=\"fixed-case\">FVB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">NJ<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\">DBA<\/jats:styled-content>\/2J, C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6J, and<jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">cByJ<\/jats:styled-content>, which have previously been characterized for acute locomotor responses to cocaine. Individual peptide profiles were analyzed using mass spectrometric profiling and principal component analysis. Sequences of assigned peptides were verified by tandem mass spectrometry. Principal component analysis classified all strains according to their distinct peptide profiles in Pit samples from adolescent mice, but not adults. Select pro\u2010opiomelanocortin\u2010derived peptides were significantly higher in adolescent<jats:styled-content style=\"fixed-case\">BALB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">cByJ<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">DBA<\/jats:styled-content>\/2J mice than in<jats:styled-content style=\"fixed-case\">FVB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">NJ<\/jats:styled-content>or C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6J mice. A subset of peptides in the<jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content>, but not in the Pit, was altered by cocaine in adolescents. A 15\u00a0mg\/kg dose of cocaine induced greater peptide alterations than a 30\u00a0mg\/kg dose, particularly in<jats:styled-content style=\"fixed-case\">FVB<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">NJ<\/jats:styled-content>animals, with larger differences in adolescents than adults. Neuropeptides in the<jats:styled-content style=\"fixed-case\">LH<\/jats:styled-content>affected by acute cocaine administration included pro\u2010opiomelanocortin\u2010, myelin basic protein\u2010, and glutamate transporter\u2010derived peptides. The observed peptide differences could contribute to differential behavioral sensitivity to cocaine among strains and ages.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13265-fig-0006-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>Peptides were measured using mass spectrometry (MALDI\u2010TOF) in individual lateral hypothalamus and pituitary samples from four strains and two ages of inbred mice in response to acute cocaine administration. Principal component analyses (PCA) classified the strains according to their peptide profiles from adolescent mice, and a subset of peptides in the lateral hypothalamus was altered by cocaine in adolescents.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13265","type":"journal-article","created":{"date-parts":[[2015,7,30]],"date-time":"2015-07-30T08:20:46Z","timestamp":1438244446000},"page":"1038-1048","source":"Crossref","is-referenced-by-count":17,"title":["Differential peptidomics assessment of strain and age differences in mice in response to acute cocaine administration"],"prefix":"10.1111","volume":"135","author":[{"given":"Elena V.","family":"Romanova","sequence":"first","affiliation":[{"name":"Department of Chemistry Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stanislav S.","family":"Rubakhin","sequence":"additional","affiliation":[{"name":"Department of Chemistry Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Ossyra","sequence":"additional","affiliation":[{"name":"Department of Psychology Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan A.","family":"Zombeck","sequence":"additional","affiliation":[{"name":"Department of Psychology Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael R.","family":"Nosek","sequence":"additional","affiliation":[{"name":"Department of Psychology Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan V.","family":"Sweedler","sequence":"additional","affiliation":[{"name":"Department of Chemistry Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin S.","family":"Rhodes","sequence":"additional","affiliation":[{"name":"Department of Psychology Beckman Institute University of Illinois at Urbana\u2010Champaign Urbana Illinois USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.105.091520"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/gbb.12081"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2003.02247.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2006.12.005"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2009.09.106"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2006.01.016"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(98)00663-5"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1385\/JMN:28:3:265"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.85.1.41"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-3806(02)00471-6"},{"key":"e_1_2_8_12_1","first-page":"115","article-title":"Protein identification by peptide mass fingerprinting","volume":"7","author":"Cottrell J. 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