{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T04:09:20Z","timestamp":1748664560892,"version":"3.41.0"},"reference-count":61,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2015,10,14]],"date-time":"2015-10-14T00:00:00Z","timestamp":1444780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["SAF\u20102013\u201044108\u2010P","FIS\u2010PI11\/01436"],"award-info":[{"award-number":["SAF\u20102013\u201044108\u2010P","FIS\u2010PI11\/01436"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008055","name":"Fundaci\u00f3n Eugenio Rodr\u00edguez Pascual","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>In search of druggable synthetic lipids that function as potential modulators of synaptic transmission and plasticity, we synthesized sulfoglycolipid<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20, which stimulates neuritic outgrowth. Here, we have explored its effects on ion channels and exocytosis in bovine chromaffin cells.<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20 augmented the rate of basal catecholamine release. Such effect did not depend on Ca<jats:sup>2+<\/jats:sup>mobilization from intracellular stores; rather,<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20\u2010elicited secretion entirely dependent on Ca<jats:sup>2+<\/jats:sup>entry through L\u2010subtype voltage\u2010activated Ca<jats:sup>2+<\/jats:sup>channels. Those channels were recruited by cell depolarization mediated by<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20 likely through its ability to enhance the recruitment of Na<jats:sup>+<\/jats:sup>channels at more hyperpolarizing potentials. Confocal imaging with fluorescent derivative<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20\u2010<jats:styled-content style=\"fixed-case\">NBD<\/jats:styled-content>revealed its rapid incorporation and confinement into the plasmalemma, supporting the idea that<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20 effects are exerted through a plasmalemmal\u2010delimited mechanism. Thus, synthetic<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20 seems to mimic several physiological effects of endogenous lipids such as regulation of ion channels, Ca<jats:sup>2+<\/jats:sup>signaling, and exocytosis. Therefore, sulfoglycolipid<jats:styled-content style=\"fixed-case\">IG<\/jats:styled-content>20 may become a pharmacological tool for investigating the role of the lipid environment on neuronal excitability, ion channels, neurotransmitter release, synaptic efficacy, and neuronal plasticity. It may also inspire the synthesis of druggable sulfoglycolipids aimed at increasing synaptic plasticity and efficacy in neurodegenerative diseases and traumatic brain\u2013spinal cord injury.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13357-fig-0011-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>The novel synthetic sulfoglycolipid IG20 mimics several physiological effects of endogenous lipids such as regulation of ion channels, Ca<jats:sup>2+<\/jats:sup>signaling, and exocytosis. This profile may eventually drive enhanced synaptic plasticity and efficacy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13357","type":"journal-article","created":{"date-parts":[[2015,9,14]],"date-time":"2015-09-14T09:11:04Z","timestamp":1442221864000},"page":"880-896","source":"Crossref","is-referenced-by-count":2,"title":["Novel synthetic sulfoglycolipid<scp>IG<\/scp>20 facilitates exocytosis in chromaffin cells through the regulation of sodium channels"],"prefix":"10.1111","volume":"135","author":[{"given":"Andrea","family":"Crespo\u2010Castrillo","sequence":"first","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Punz\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"de Pascual","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Departamento de Farmacolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcos","family":"Maroto","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Fernando","family":"Pad\u00edn","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Departamento de Farmacolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabel","family":"Garc\u00eda\u2010\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Hospital Nacional de Parapl\u00e9jicos SESCAM Toledo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Nanclares","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Departamento de Farmacolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"Ruiz\u2010Pascual","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis","family":"Gand\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Departamento de Farmacolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfonso","family":"Fern\u00e1ndez\u2010Mayoralas","sequence":"additional","affiliation":[{"name":"Instituto de Qu\u0269mica Org\u00e1nica General CSIC Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio G.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto Te\u00f3filo Hernando Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Departamento de Farmacolog\u00eda Facultad de Medicina Universidad Aut\u00f3noma de Madrid Madrid Spain"},{"name":"Servicio de Farmacolog\u00eda Cl\u00ednica Instituto de Investigaci\u00f3n Sanitaria Hospital Universitario de La Princesa Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.144.2.241"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biophys.36.040306.132643"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbiomolbio.2009.01.012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503465103"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.35.21100"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-0270(86)90054-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0704839"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.10.006"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.1980.60.2.396"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00364.2002"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1745"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2009.04.024"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2010.191585"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.200600195"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.11.070"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0809847106"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm0611556"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(02)00370-7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.2002.00997.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-4388(96)80119-X"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704084200"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.89.129"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222003110"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05521.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-15-06507.2002"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03403.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.108.110817"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01465-06"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.1981.tb01724.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80319-C"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00185a021"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M809737200"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020496"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.200304822"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47340-7"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-178-9_23"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/glia.20292"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/0926-6917(94)90009-4"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)01543-4"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1985.sp015817"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90287-J"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth782"},{"key":"e_1_2_7_45_1","unstructured":"Nieto\u2010SampedroM. Fern\u00e1ndez\u2010MayoralasA. Doncel\u2010P\u00e9rezE.andGarc\u00eda\u2010AlvarezI.(2012)Use of sulphated glycolipids as promoters of neuritic growth and myelination and inhibitors of the proliferation of astroglia and microglia. Spanish Patent P201231045. Spain."},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00916-9"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304409200"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.08.084"},{"key":"e_1_2_7_49_1","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/S0022-3565(25)29393-4","article-title":"Effects of veratrum alkaloids on membrane potential and conductance of squid and crayfish giant axons","volume":"184","author":"Ota M.","year":"1973","journal-title":"J. Pharmacol. Exp. Ther."},{"key":"e_1_2_7_50_1","first-page":"133","article-title":"Thapsigargine and thapsigargicine, two new histamine liberators from Thapsia garganica L","volume":"15","author":"Rasmussen U.","year":"1978","journal-title":"Acta Pharm. Suec."},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.54.3.431"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1146-04.2004"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-010-0861-x"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/42408"},{"key":"e_1_2_7_55_1","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0031-6997(25)00447-8","article-title":"Cardiac alpha 1\u2010adrenoceptors: an overview","volume":"45","author":"Terzic A.","year":"1993","journal-title":"Pharmacol. Rev."},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3715-12.2012"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874467208666150507105443"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.283374"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.0421v.x"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199801000-00019"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)80642-4"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2014.06.007"}],"container-title":["Journal of Neurochemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.13357","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjnc.13357","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jnc.13357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T16:24:35Z","timestamp":1748622275000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jnc.13357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,14]]},"references-count":61,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2015,12]]}},"alternative-id":["10.1111\/jnc.13357"],"URL":"https:\/\/doi.org\/10.1111\/jnc.13357","archive":["Portico"],"relation":{},"ISSN":["0022-3042","1471-4159"],"issn-type":[{"type":"print","value":"0022-3042"},{"type":"electronic","value":"1471-4159"}],"subject":[],"published":{"date-parts":[[2015,10,14]]}}}