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In status epilepticus models alterations to<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>subunit expression have been suggested to contribute to (i) abnormal development of the<jats:styled-content style=\"fixed-case\">GABA<\/jats:styled-content>ergic system, (ii) why seizures become self\u2010sustaining and (iii) the development of pharmacoresistance. Detailed investigation of<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>subunit protein expression after neonatal hypoxia\u2010ischaemia (<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>) is currently insufficient. Using our pig model of<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>and subsequent spontaneous neonatal seizures, we investigated changes in protein expression of the three predominant \u03b1\u2010subunits of the<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>; \u03b1<jats:sub>1<\/jats:sub>, \u03b1<jats:sub>2<\/jats:sub>and \u03b1<jats:sub>3<\/jats:sub>. Anaesthetized, ventilated newborn pigs (&lt;\u00a024\u00a0h old) were subjected to 30\u00a0min<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>and subsequently recovered to 24 or 72\u00a0h. Amplitude\u2010integrated electroencephalography was used to monitor brain activity and identify seizure activity. Brain tissue was collected post\u2010mortem and<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>\u03b1\u2010subunit protein expression was analysed using western blot and immunohistochemistry.<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>\u03b1<jats:sub>1<\/jats:sub>and \u03b1<jats:sub>3<\/jats:sub>protein expression was significantly reduced in animals that developed seizures after<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>;<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>animals that did not develop seizures did not exhibit the same reductions. Immunohistochemistry revealed decreased \u03b1<jats:sub>1<\/jats:sub>and \u03b1<jats:sub>3<\/jats:sub>expression, and \u03b1<jats:sub>1<\/jats:sub>redistribution from the cell membrane to the cytosol, in the hippocampus of seizure animals. Multivariate analyses, controlling for<jats:styled-content style=\"fixed-case\">HI<\/jats:styled-content>severity and neuronal injury, revealed that seizures were independently associated with significant<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>\u03b1<jats:sub>3<\/jats:sub>reduction. This is the first study to show loss and redistribution of<jats:styled-content style=\"fixed-case\">GABA<jats:sub>A<\/jats:sub>R<\/jats:styled-content>\u03b1\u2010subunits in a neonatal brain experiencing seizures. Our findings are similar to those reported in models of<jats:styled-content style=\"fixed-case\">SE<\/jats:styled-content>and in chronic epilepsy.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13746-fig-0006-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13746","type":"journal-article","created":{"date-parts":[[2016,7,26]],"date-time":"2016-07-26T11:53:57Z","timestamp":1469534037000},"page":"471-484","source":"Crossref","is-referenced-by-count":23,"title":["Neonatal seizures are associated with redistribution and loss of<scp>GABA<sub>A<\/sub><\/scp>\u03b1\u2010subunits in the\u00a0hypoxic\u2010ischaemic pig"],"prefix":"10.1111","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5565-2001","authenticated-orcid":false,"given":"Stephanie M.","family":"Miller","sequence":"first","affiliation":[{"name":"The University of Queensland Perinatal Research Centre UQ Centre for Clinical Research Herston Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"The University of Queensland Perinatal Research Centre UQ Centre for Clinical Research Herston Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zoe","family":"Ireland","sequence":"additional","affiliation":[{"name":"The University of Queensland Perinatal Research Centre UQ Centre for Clinical Research Herston Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7739-2098","authenticated-orcid":false,"given":"Kirat K.","family":"Chand","sequence":"additional","affiliation":[{"name":"The University of Queensland Perinatal Research Centre UQ Centre for Clinical Research Herston Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6408-8238","authenticated-orcid":false,"given":"Paul B.","family":"Colditz","sequence":"additional","affiliation":[{"name":"The University of Queensland Perinatal Research Centre UQ Centre for Clinical Research Herston Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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