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They have also served as invaluable models to study the kinetics of single\u2010vesicle exocytotic events to infer the characteristics of opening and closing of the exocytotic fusion pore. We have here tested the hypothesis that stimulation at room temperature of<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>s from mice C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 with physiological acetylcholine (<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h) and with other secretagogues (dimethylphenylpiperazinium, high K<jats:sup>+<\/jats:sup>, muscarine, histamine, caffeine), alone or in combination, could trigger amperometric spike events with different kinetics. We found that mean secretory spike events in<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>s stimulated with<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h had a fast rise rate of 25<jats:styled-content style=\"fixed-case\">pA<\/jats:styled-content>\/ms and a rapid decay time of 6.2\u00a0ms, with a small quantal size (0.31<jats:styled-content style=\"fixed-case\">pC<\/jats:styled-content>). Surprisingly, these parameters considerably differed from those found in<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>s stimulated with all other secretagogues that triggered secretory responses with spike events having smaller rise rates, longer decay times and higher quantal sizes.<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h spikes were unaltered by atropine but mitochondrial protonophore carbonyl cyanide\u2010<jats:italic>4<\/jats:italic>\u2010(trifluoromethoxy)phenylhydrazone markedly slowed down the rate rise and decay time, and augmented the quantal size of mean secretory events. We conclude that the physiological neurotransmitter<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>h triggers a fast and efficient exocytotic response that cannot be mimicked by other secretagogues; such response is regulated by the mitochondrial circulation of calcium ions.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p><jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13849-fig-0007-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text><\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13849","type":"journal-article","created":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T07:50:54Z","timestamp":1474444254000},"page":"722-736","source":"Crossref","is-referenced-by-count":16,"title":["Faster kinetics of quantal catecholamine release in mouse chromaffin cells 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