{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T10:49:42Z","timestamp":1790678982485,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,11,10]],"date-time":"2016-11-10T00:00:00Z","timestamp":1478736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005294","name":"Massachusetts General Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005294","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["WE 5324\/1\u20101"],"award-info":[{"award-number":["WE 5324\/1\u20101"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Neurochemistry"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:label\/><jats:p>Tau is a neuronal microtubule\u2010binding protein that, in Alzheimer's disease and other neurodegenerative diseases, can form oligomeric and large fibrillar aggregates, which deposit in neurofibrillary tangles. Tau's physiological state of multimerization appears to vary across conditions, and a stable dimeric form of soluble tau has been suggested from experiments using recombinant tau <jats:italic>in\u00a0vitro<\/jats:italic>. We tested if tau dimerization or oligomerization, also occurs in cells, and if soluble tau oligomers are relevant for the release and internalization of tau. We developed a sensitive tau split\u2010luciferase assay to show the rapid intracellular formation of stable tau dimers that are released and taken up by cells. Our data further suggest that tau dimerization can be accelerated slightly by aggregation catalysts. We conclude that tau oligomers are a stable physiological form of tau, and that tau oligomerization does not necessarily lead to tau aggregation.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13866-fig-0006-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13866","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T20:35:43Z","timestamp":1476390943000},"page":"1163-1174","source":"Crossref","is-referenced-by-count":61,"title":["Formation, release, and internalization of stable tau oligomers in cells"],"prefix":"10.1111","volume":"139","author":[{"given":"Susanne","family":"Wegmann","sequence":"first","affiliation":[{"name":"Department of Neurology Massachusetts General Hospital MassGeneral Institute of Neurodegenerative Diseases (MIND) Charlestown Massachusetts USA"},{"name":"Department of Neurology Harvard Medical School Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samantha","family":"Nicholls","sequence":"additional","affiliation":[{"name":"Department of Neurology Massachusetts General Hospital MassGeneral Institute of Neurodegenerative Diseases (MIND) Charlestown Massachusetts USA"},{"name":"Department of Neurology Harvard Medical School Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuko","family":"Takeda","sequence":"additional","affiliation":[{"name":"Department of Neurology Massachusetts General Hospital MassGeneral Institute of Neurodegenerative Diseases (MIND) Charlestown Massachusetts USA"},{"name":"Department of Neurology Harvard Medical School Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhanyun","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Neurology Massachusetts General Hospital MassGeneral Institute of Neurodegenerative Diseases (MIND) Charlestown Massachusetts USA"},{"name":"Department of Neurology Harvard Medical School Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bradley T.","family":"Hyman","sequence":"additional","affiliation":[{"name":"Department of Neurology Massachusetts General Hospital MassGeneral Institute of Neurodegenerative Diseases (MIND) Charlestown Massachusetts USA"},{"name":"Department of Neurology Harvard Medical School Boston Massachusetts USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi026469j"},{"key":"e_1_2_6_3_1","first-page":"35","article-title":"Purification of recombinant tau protein and preparation of Alzheimer\u2010paired helical filaments in\u00a0vitro","volume":"299","author":"Barghorn S.","year":"2005","journal-title":"Methods Mol. 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