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Herein, we utilized luminescent quantum dots (<jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>s) conjugated to margatoxin (MgTx) as a traceable vehicle to target a voltage\u2010dependent potassium channel, Kv1.3, which has a select distribution and well\u2010characterized role in immunity, glucose metabolism, and sensory ability. We screened both unconjugated (MgTx) and conjugated MgTx (<jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>\u2010MgTx) for their ability to inhibit <jats:italic>Shaker<\/jats:italic> channels Kv1.1 to Kv1.7 using patch\u2010clamp electrophysiology in <jats:styled-content style=\"fixed-case\">HEK<\/jats:styled-content>293 cells. Our data indicate that MgTx inhibits 79% of the outward current in Kv1.3\u2010transfected cells and that the <jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>\u2010MgTx conjugate is able to achieve a similar level of block, albeit a slightly reduced efficacy (66%) and at a slower time course (50% block by 10.9\u00a0\u00b1\u00a01.1\u00a0min, MgTx; vs. 15.3\u00a0\u00b1\u00a01.2\u00a0min, <jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>\u2010MgTx). Like the unbound peptide, the <jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>\u2010MgTx conjugate inhibits both Kv1.3 and Kv1.2 at a 1\u00a0nM concentration, whereas it does not inhibit other screened <jats:italic>Shaker<\/jats:italic> channels. We tested the ability of <jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>\u2010MgTx to inhibit native Kv1.3 expressed in the mouse olfactory bulb (OB). In brain slices of the OB, the conjugate acted similarly to MgTx to inhibit Kv1.3, causing an increased action potential firing frequency attributed to decreased intraburst duration rather than interspike interval. Our data demonstrate a retention of known biophysical properties associated with block of the vestibule of Kv1.3 by <jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>\u2010MgTx conjugate compared to that of MgTx, inferring <jats:styled-content style=\"fixed-case\">QD<\/jats:styled-content>s could provide a useful tool to deliver ion channel inhibitors to targeted tissues <jats:italic>in\u00a0vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>\n<jats:boxed-text content-type=\"graphic\" position=\"anchor\"><jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" mimetype=\"image\/png\" position=\"anchor\" specific-use=\"enlarged-web-image\" xlink:href=\"graphic\/jnc13891-fig-0008-m.png\"><jats:alt-text>image<\/jats:alt-text><\/jats:graphic><\/jats:boxed-text>\n<\/jats:p><\/jats:sec>","DOI":"10.1111\/jnc.13891","type":"journal-article","created":{"date-parts":[[2016,11,8]],"date-time":"2016-11-08T16:17:42Z","timestamp":1478621862000},"page":"404-420","source":"Crossref","is-referenced-by-count":16,"title":["Margatoxin\u2010bound quantum dots as a novel inhibitor of the voltage\u2010gated ion channel Kv1.3"],"prefix":"10.1111","volume":"140","author":[{"given":"Austin B.","family":"Schwartz","sequence":"first","affiliation":[{"name":"Institute of Molecular Biophysics Florida State University Tallahassee Florida USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anshika","family":"Kapur","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Florida State University Tallahassee Florida 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